Regulation of cardiac mesodermal and neural crest development by the bHLH transcription factor, dHAND
Regulation of cardiac mesodermal and neural crest development by the bHLH transcription factor, dHAND
复制标题
DOI：
10.1038/ng0697-154
复制
发表时间：
1997-06-01
期刊：
影响因子：
30.8
通讯作者：
Olson, EN
中科院分区：
文献类型：
--
作者：
Srivastava, D;Thomas, T;Olson, EN
DHAND和eHAND是相关的碱性螺旋-环-螺旋(BHLH)转录因子，在发育中的心脏的中胚层和神经脊来源的结构中表达。与它们在鸟类心脏发生过程中的同源表达不同，在小鼠心脏发育过程中，我们发现DHAND和eHAND以互补的方式表达，并分别局限于注定要形成右心室和左心室的心管部分。DHAND和eHAND是迄今发现的最早的心腔特异转录因子。小鼠胚胎中靶基因DHAND的缺失导致了胚胎在第10.5天因心力衰竭而死亡。我们对DHAND突变胚胎心脏表型的描述首次证明了控制中胚层来源的右室和神经脊源的主动脉弓形成的单个基因，并揭示了右室发育的一种新的心源性亚型。
dHAND and eHAND are related basic helix-loop-helix (bHLH) transcription factors that are expressed in mesodermal and neural crest-derived structures of the developing heart. In contrast to their homogeneous expression during avian cardiogenesis, during mouse heart development we show that dHAND and eHAND are expressed in a complementary fashion and are restricted to segments of the heart tube fated to form the right and left ventricles, respectively. dHAND and eHAND represent the earliest cardiac chamber-specific transcription factors yet identified. Targeted gene deletion of dHAND in mouse embryos resulted in embryonic lethality at embryonic day 10.5 from heart failure. Our description of the cardiac phenotype of dHAND mutant embryos is the first demonstration of a single gene controlling the formation of the mesodermally derived right ventricle and the neural crest-derived aortic arches and reveals a novel cardiogenic subprogramme for right ventricular development.