Upregulation of B7.2, but not B7.1, on B cells from patients with allergic asthma
Upregulation of B7.2, but not B7.1, on B cells from patients with allergic asthma
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DOI：
10.1016/s0091-6749(98)70199-x
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发表时间：
1998-01-01
影响因子：
14.2
通讯作者：
Leung, DYM
中科院分区：
文献类型：
--
作者：
Hofer, MF;Jirapongsananuruk, O;Leung, DYM
背景：过敏性哮喘与T-H2样细胞反应和IgE产生增加有关。最近在小鼠中的研究表明共刺激分子B7.2（CD 86）可能影响T-H2细胞的发育。目的：我们试图确定B7.2在哮喘患者中的潜在作用。方法：我们使用五参数流式细胞术分析来自过敏性哮喘患者和健康对照受试者的B细胞上B7.1和B7.2的表达。我们报告，特应性哮喘患者暴露于过敏原，（p < 0.005）在B细胞上的B7.2表达水平高于在体内未暴露于变应原的特应性哮喘受试者或非特应性对照受试者。相反，在三个研究受试者组中B7.1（CD 80）表达没有差异，当哮喘患者和正常对照者的外周血单个核细胞用IL-4或IL-13刺激时，B7.2的表达显著增加，但B7.1的表达不显著（p < 0.005）在B细胞上，干扰素γ或IL-12不影响这两种分子的表达，IL-4诱导B7.2在过敏性疾病中的功能意义是通过该分子在CD 23（+）上的表达增加来表明的，但不是CD 23（-），B细胞。结论：这些结果表明，同一B细胞参与过敏原的介绍也表达共刺激分子B7.2，并支持这一假设，即该分子是一个重要的共刺激分子在过敏反应，其表达可以调节T-H2样细胞因子。
Background: Allergic asthma is associated with T-H2-like cell responses and increased IgE production. Recent studies in mice have suggested that the costimulatory molecule B7.2 (CD86) may influence the development of T-H2 cells.Objective: We sought to determine the potential role of B7.2 in patients with asthma.Methods: We performed an analysis of B cells from patients with allergic asthma and healthy control subjects for expression of B7.1 and B7.2 on B cells using five-parameter flow cytometry,Results: We report that atopic patients with asthma who are exposed to allergens have significantly (p < 0.005) higher levels of B7.2 expression on B cells than atopic asthmatic subjects not exposed to allergen in vivo or nonatopic control subjects, In contrast, there vc ere no differences in B7.1 (CD80) expression among the three study subject groups, When peripheral blood mononuclear cells from asthmatic patients or normal control subjects were stimulated with IL-4 or IL-13, the expression of B7.2, but not B7.1, was significantly increased (p < 0.005) on B cells, Interferon-gamma or IL-12 did not affect the expression of either molecule, The functional significance of B7.2 induction by IL-4 in allergic disease was suggested by the increased expression of this molecule on CD23(+), but not CD23(-), B cells.Conclusion: These results indicate that the same B cell involved in allergen presentation also expresses the costimulatory molecule B7.2 and support the hypothesis that this molecule is an important costimulatory molecule in allergic responses, the expression of which can be modulated by T-H2-like cytokines.