INTESTINAL CRYPT PROLIFERATION .2. COMPUTER MODELING OF MITOTIC INDEX DATA PROVIDES FURTHER EVIDENCE FOR LATERAL AND VERTICAL CELL-MIGRATION IN THE ABSENCE OF MITOTIC-ACTIVITY
INTESTINAL CRYPT PROLIFERATION .2. COMPUTER MODELING OF MITOTIC INDEX DATA PROVIDES FURTHER EVIDENCE FOR LATERAL AND VERTICAL CELL-MIGRATION IN THE ABSENCE OF MITOTIC-ACTIVITY
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DOI：
10.1111/j.1365-2184.1988.tb00784.x
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发表时间：
1988-07-01
期刊：
影响因子：
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通讯作者：
CHWALINSKI, S
中科院分区：
文献类型：
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作者：
LOEFFLER, M;POTTEN, CS;CHWALINSKI, S
对长春新碱给药前、给药后1、2和3 h的位置依赖性有丝分裂指数进行评分。细胞在有丝分裂中的积累导致在隐窝位置8-12处有丝分裂指数从0.06增加到0.34。令人惊讶的是，位置相关的有丝分裂指数分布的前沿移动到更高的隐窝位置，虽然细胞分裂停止。此外，还记录了切片中有丝分裂像的垂直聚集。使用先前描述的计算机隐窝模型检查数据。我们得出结论：平均有丝分裂期持续时间约为0.7小时（40分钟），并且随细胞位置变化很小;在隐窝切片中，用于过度计算有丝分裂的几何校正因子约为0.6-0.7，并且相邻的细胞柱可以合并。有丝分裂后的细胞横向位移，如先前的模型分析所预测的，将是一种机制，以抵消其他力量，往往减少隐窝周长。在正常的稳定状态下，合并和膨胀过程会彼此平衡。如果其中一个机制受阻，就不会出现这种情况。因此，我们提出了一个新的概念，其中的隐窝几何形状将动态地确定与新细胞的横向定位，一方面和另一方面的收缩力的细胞增殖活性。
The position-dependent mitotic index before, and 1, 2 and 3 h after vincristine was scored. The accumulation of cells in mitosis leads to an increase in the mitotic index from 0.06 to 0.34 at crypt positions 8-12. Surprisingly, the leading edge of the position-related mitotic index distribution moves to higher crypt positions although cell division was stopped. In addition, the vertical clustering of mitotic figures in sections was recorded. The data were examined using a previously described computer crypt model. We conclude: the average mitotic phase duration is about 0.7 h (40 min) and varies little with cell position; the geometrical correction factor for overscoring mitoses in crypt sections is about 0.6-0.7 and adjacent cell columns can merge. Lateral cell displacement after mitosis, as predicted in a previous model analysis, would be a mechanism to counteract other forces that tend to reduce the crypt circumference. In the normal steady state merging and expansion processes would just balance each other. This would not follow if one mechanism was blocked. Thus we propose a new concept in which the crypt geometry would be dynamically determined by cell proliferative activity in connection with lateral positioning of new cells on one hand and contracting forces on the other hand.