Androgen receptor, aromatase, oestrogen receptor α/β and G protein-coupled receptor 30 expression in the testes and epididymides of adult sheep
Androgen receptor, aromatase, oestrogen receptor α/β and G protein-coupled receptor 30 expression in the testes and epididymides of adult sheep
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成年羊睾丸和附睾中雄激素受体、芳香酶、雌激素受体α/β和G蛋白偶联受体30的表达
DOI：
10.1111/rda.13638
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发表时间：
2020-02-17
影响因子：
1.7
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Ge, Wenbo;Xiao, Longfei;Zhang, Yong
雄激素受体（AR）在生殖中起关键作用，芳香化酶（P450 arom）、核雌激素受体（ER）α和β以及G蛋白偶联受体30（GPR 30）对睾丸和附睾细胞增殖和发育都很重要。本研究采用定量逆转录-聚合酶链反应（RT-PCR）、Western blotting和免疫组织化学方法，对AR、P450 arom、ER α、ER β和GPR 30在性成熟绵羊睾丸和附睾中的表达和定位进行了研究。结果表明，附睾头和附睾体的AR、P450 arom和ER α水平显著低于睾丸和附睾尾睾丸ER β水平显著高于附睾头、体、尾（p <0.05）（p <0.05），附睾头中的GPR 30水平显著低于睾丸和附睾体和尾（p <0.05）。这些受体主要在附睾上皮、基底层、平滑肌、支持细胞和间质细胞以及精子中检测到。总之，结果表明，绵羊附睾和睾丸具有合成雌二醇的潜力，是雄激素和雌二醇的靶点。这些结果为进一步研究绵羊睾丸和附睾中雄激素和雌二醇信号转导机制奠定了基础。
The androgen receptor (AR) plays a key role in reproduction, and aromatase (P450arom), nuclear oestrogen receptors (ERs) alpha and beta, and G protein-coupled receptor 30 (GPR30) are important for testicular and epididymal cell proliferation and development. In the study, we have investigated the expression and localization of AR, P450arom, ER alpha, ER beta and GPR30 in testes and epididymides of sexually mature sheep by quantitative reverse transcription-polymerase chain reaction, Western blotting and immunohistochemistry. The results demonstrate that the AR, P450arom and ER alpha levels in the caput and corpus epididymis were significantly lower than those in the testis and cauda epididymis (p < .05), the ER beta level in the testis was significantly higher than in the caput, corpus and cauda epididymis (p < .05), and the GPR30 level in the caput epididymis was significantly lower than in the testis and corpus and cauda epididymis (p < .05). These receptors were mainly detected in epididymal epithelial, basal, smooth muscle, Sertoli and Leydig cells, as well as in spermatozoa. Taken together, the results suggest that sheep epididymides and testes have the potential for estradiol synthesis and are the targets of both androgens and estradiol. These results provide a foundation for further studies on the mechanisms of androgens and estradiol signalling in the testes and epididymides of sheep.