Ferrophilic characteristics of Vibrio vulnificus and potential usefulness of iron chelation therapy
Ferrophilic characteristics of Vibrio vulnificus and potential usefulness of iron chelation therapy
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DOI：
10.1086/509822
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发表时间：
2007-01-01
影响因子：
6.4
通讯作者：
Shin, Sung-Heui
中科院分区：
文献类型：
--
作者：
Kim, Choon-Mee;Park, Ra-Young;Shin, Sung-Heui
我们确定了创伤弧菌的亲铁特性，以评估铁螯合治疗对预防创伤弧菌感染的潜在有效性。易获得的非转铁蛋白结合铁（NTBI）是在体外铁限制条件下和人离体条件下启动创伤弧菌生长所必需的。NTBI有助于创伤弧菌有效利用转铁蛋白结合铁（TBI），并且在NTBI启动细菌生长后，vulnibactin介导的铁摄取系统得以表达。创伤弧菌需要较高的NTBI水平来开始生长，产生较低水平的铁载体，并且比其他细菌更有效地利用TBI。此外，临床可用的铁螯合剂去铁素可抑制创伤弧菌的生长。这些结果表明，创伤弧菌是一种亲铁细菌，比其他病原体需要更高的NTBI水平，铁螯合治疗可能是防止易感患者创伤弧菌体内生长的有效手段。
We determined the ferrophilic characteristics of Vibrio vulnificus to evaluate the potential usefulness of iron chelation therapy for the prevention of V. vulnificus infection. Readily available non-transferrin-bound iron (NTBI) is required for the initiation of V. vulnificus growth under in vitro iron-limited conditions and human ex vivo conditions. NTBI aided efficient transferrin-bound iron (TBI) use by V. vulnificus, and the vulnibactin-mediated iron-uptake system was expressed after bacterial growth had been started by NTBI. V. vulnificus required higher NTBI levels for the initiation of growth, produced siderophores at lower levels, and used TBI less efficiently than other bacteria. In addition, the growth of V. vulnificus was inhibited by deferiprone, a clinically available iron chelator. These results show that V. vulnificus is a ferrophilic bacterium that requires higher NTBI levels than other pathogens and that iron chelation therapy might be an effective means of preventing the in vivo growth of V. vulnificus in susceptible patients.