GENOMIC SEQUENCE SAMPLING - A STRATEGY FOR HIGH-RESOLUTION SEQUENCE-BASED PHYSICAL MAPPING OF COMPLEX GENOMES
GENOMIC SEQUENCE SAMPLING - A STRATEGY FOR HIGH-RESOLUTION SEQUENCE-BASED PHYSICAL MAPPING OF COMPLEX GENOMES
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DOI：
10.1038/ng0594-40
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发表时间：
1994-05-01
期刊：
影响因子：
30.8
通讯作者：
EVANS, GA
中科院分区：
文献类型：
--
作者：
SMITH, MW;HOLMSEN, AL;EVANS, GA
我们介绍了一种基于采样测序构建大面积基因组 DNA 高分辨率物理图谱的简单而高效的方法。物理图谱的构建方法是对高密度宇宙比对序列进行排序，并确定每个克隆两端的序列片段。由此绘制的图谱包含 30-50% 的完整 DNA 序列，可以识别许多基因，并能对基因组的几乎任何部分进行 PCR 扩增。我们将这一策略应用于原始真核生物蓝氏贾第鞭毛虫基因组的自动分析，并对其在人类基因组的物理图谱绘制和 DNA 测序中的适用性进行了评估。
We present a simple and efficient method for constructing high resolution physical maps of large regions of genomic DNA based upon sampled sequencing. The physical map is constructed by ordering high density cosmid contigs and determining a sequence fragment from each end of every clone. The resulting map, which contains 30-50% of the complete DNA sequence, allows the identification of many genes and makes possible PCR amplification of virtually any part of the genome. We apply this strategy to the automated analysis of the genome of the primitive eukaryote Giardia lamblia and evaluate its applicability to the physical mapping and DNA sequencing of the human genome.