Comparison between immunofluorescence and immunomagnetic techniques of cytometry
Comparison between immunofluorescence and immunomagnetic techniques of cytometry
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免疫荧光与免疫磁珠细胞术技术的比较
DOI：
10.1016/s0304-8853(98)00567-8
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发表时间：
1999
影响因子：
2.7
通讯作者：
M. Krönke
中科院分区：
文献类型：
--
作者：
V. Tchikov;S. Schütze;M. Krönke
磁电泳和荧光激活细胞分选用于评价磁性颗粒和荧光探针的免疫化学性质。用一抗和二抗与荧光分子或磁性颗粒偶联检测REH细胞表面的HLA-Bw 6抗原。磁泳可以在生物学和医学中应用于测量细胞亚群的百分比。
Magnetophoresis and fluorescence activated cell sorting were used for evaluation of immunochemical properties of magnetic particles and fluorescent probes. The HLA-Bw6 antigen on surfaces of REH cells was detected with a primary monoclonal antibody and a secondary antibody coupled with fluorescent molecules or magnetic particles. Magnetophoresis can find applications in biology and medicine for measuring percentages of cell subpopulations.