(6-4)PHOTOPRODUCTS ARE REMOVED FROM THE DNA OF UV-IRRADIATED MAMMALIAN-CELLS MORE EFFICIENTLY THAN CYCLOBUTANE PYRIMIDINE DIMERS
(6-4)PHOTOPRODUCTS ARE REMOVED FROM THE DNA OF UV-IRRADIATED MAMMALIAN-CELLS MORE EFFICIENTLY THAN CYCLOBUTANE PYRIMIDINE DIMERS
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DOI：
10.1016/s0165-7992(85)80018-x
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
CLARKSON, JM
中科院分区：
文献类型：
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作者：
MITCHELL, DL;HAIPEK, CA;CLARKSON, JM
通过改变32p标记抗原的性质，建立了一种针对紫外线照射DNA的多克隆抗血清，可特异性检测环丁烷嘧啶二聚体和Pyr(6-4)Pyo光产物。在紫外线照射的人、仓鼠和小鼠细胞中，Pyr(6-4)Pyo光产物的去除速度比环丁烷二聚体快。互补组A、C和D的着色性干皮细胞对两种病变的修复都有缺陷。
A polyclonal antiserum raised against UV-irradiated DNA can be used to assay cyclobutane pyrimidine dimers and Pyr(6-4)Pyo photoproducts specifically by changing the nature of the 32P-labeled antigen. Pyr(6-4)Pyo photoproducts were removed faster than cyclobutane dimers in UV-irradiated human, hamster and mouse cells. Xeroderma pigmentosum cells from complementation groups A, C and D were deficient in the repair of both lesions.