Profiling of differentially expressed genes in cadmium-induced prostate carcinogenesis.
Profiling of differentially expressed genes in cadmium-induced prostate carcinogenesis.
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DOI：
10.1016/j.taap.2019.05.008
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发表时间：
2019-07
影响因子：
3.8
通讯作者：
Venkatesh Kolluru;A. Tyagi;Balaji Chandrasekaran;C. Damodaran
中科院分区：
文献类型：
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作者：
Venkatesh Kolluru;A. Tyagi;Balaji Chandrasekaran;C. Damodaran
本研究的目的是调查镉转化的前列腺上皮细胞（CTPE）的遗传特征，并确定其恶性转化中涉及的潜在分子信号。数据集包含正常前列腺上皮细胞（RWPE-1）和CTPE细胞。为了进一步检查鉴定的差异表达基因（DEG）的生物学功能，进行基因本体（GO）、京都基因和基因组百科全书（KEGG）和Reactome途径富集分析。总共鉴定了2357个DEG，包括1083个上调基因和1274个下调基因。GO、KEGG和Reactome途径富集分析表明，上调基因显著富集于ECM受体、粘着斑、TGFβ信号传导和多配体蛋白聚糖相互作用，而下调基因主要涉及细胞周期调控、花生四烯酸代谢、氧化磷酸化和叶酸生物合成（p<0.05）。通过qRT-PCR分析进一步验证了顶部上调（SATB 1（p<.0001）、EYA 2（p < .0001）和KPNA 7（p<.0027））和下调（PITX 2（p< .0007）、PDLIM 4（p< .0020）和FABP 5（p < .0007））的基因。总之，本研究分析了RWPE-1和CTPE细胞中的DEG，并鉴定了可能与恶性转化和肿瘤进展相关的基因通路。
The aim of the present study was to investigate the genetic signatures of cadmium-transformed prostate epithelial (CTPE) cells and to identify the potential molecular signaling involved in their malignant transformation. The dataset contained normal prostate epithelial (RWPE-1) and CTPE cells. To further examine the biological functions of the identified differentially expressed genes (DEGs), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome pathway enrichment analyses were performed. In total, 2357 DEGs were identified, including 1083 upregulated genes and 1274 downregulated genes. GO, KEGG, and Reactome pathway enrichment analyses indicated that upregulated genes were significantly enriched in ECM-receptor, focal adhesion, TGFβ signaling, and syndecan interactions, while downregulated genes were mainly involved in cell cycle regulation, arachidonic acid metabolism, oxidative phosphorylation, and folate biosynthesis (p< .05). The top upregulated (SATB1 (p< .0001), EYA2 (p < .0001) and KPNA7 (p< .0027)) and downregulated (PITX2 (p< .0007), PDLIM4 (p< .0020) and FABP5 (p < .0007)) genes were further validated via qRT-PCR analysis. In conclusion, the present study profiled DEGs in RWPE-1 and CTPE cells and identified gene pathways that may be associated with malignant transformation and tumor progression.