Detection of T suppressor cells in patients with organ allografts
Detection of T suppressor cells in patients with organ allografts
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DOI：
10.1016/s0198-8859(00)00226-3
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发表时间：
2001-01-01
期刊：
影响因子：
2.7
通讯作者：
Cortesini, NSF
中科院分区：
文献类型：
--
作者：
Ciubotariu, R;Vasilescu, R;Cortesini, NSF
特异性免疫抑制宿主对供者的免疫应答一直是临床移植的主要目标。最近的证据表明，表达CHE CD8(+)CD28(-)表型的T细胞具有抑制功能，并通过调节APC功能来抑制Th的激活和增殖。为了评估移植受者循环中TS的存在，我们建立了一种流式细胞术方法来检测受体Th和Ts暴露下供者APC上共刺激分子的表达。我们的结果表明，定量检测患者循环中CD8(+)CD28(-)T细胞抑制供者APC上共刺激分子(CD80、CD86)激活的能力为监测实体器官移植中针对移植物的特异性免疫抑制提供了可靠的工具。《人类免疫学》，第62期，第15-20期(2001)。(C)美国组织相容性和免疫遗传学会，2001年。爱思唯尔科学公司出版。
Specific immunosuppression of host's immune response to donor HLA antigens has been a major goal to clinical transplantation. Recent evidence has been accumulating to show that a distinct population of T cells expressing che CD8(+) CD28(-) phenotype display suppressor function and inhibit Th activation and proliferation by modulating the APC function. To assess the presence of Ts in transplant recipient's circulation, we have developed a flow cytometry method that measures the expression of costimulatory molecules on donor APC exposed to recipient Th and Ts, Our results demonstrate that quantitation of the capacity of CD8(+) CD28(-) T cells from patient circulation to suppress the activation of costimulatory molecules (CD80, CD86) on donor APC offers a reliable tool for monitoring specific immunosuppression against the graft in solid organ transplantation. Human Immunology 62, 15-20 (2001). (C) American Society for Histocompatibility and Immunogenetics, 2001. Published by Elsevier Science Inc.