Crystallization and preliminary crystallographic data of SnoaL, a polyketide cyclase in nogalamycin biosynthesis.
Crystallization and preliminary crystallographic data of SnoaL, a polyketide cyclase in nogalamycin biosynthesis.
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DOI：
10.1107/s090744490400705x
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发表时间：
2004-06
期刊：
影响因子：
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通讯作者：
A. Sultana;P. Kallio;A. Jansson;J. Niemi;P. Mäntsälä;G. Schneider
中科院分区：
文献类型：
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作者：
A. Sultana;P. Kallio;A. Jansson;J. Niemi;P. Mäntsälä;G. Schneider
诺丙酸甲酯环化酶(SnoaL)催化聚酮类抗生素诺加拉霉素生物合成的最后一步合环反应。以聚乙二醇4000为沉淀剂，合成了SnoaL与底物诺麦隆酸甲酯的络合物晶体。晶体属正交晶系，空间群为I222，晶胞参数a=69.1，b=72.0，c=65.4埃。它们使用同步辐射衍射到1.35埃的分辨率。马修斯系数2.0埃3 Da(-1)表明不对称单位中有一个亚基。收集了一种同构铀衍生物的衍射数据，差分Patterson图显示了可以确定铀离子位置的强峰。
Nogalonic acid methyl ester cyclase (SnoaL) catalyzes the last ring-closure step in the biosynthesis of the polyketide antibiotic nogalamycin. Crystals of a complex of SnoaL with the substrate nogalonic acid methyl ester have been obtained using PEG 4000 as precipitant. The crystals are orthorhombic, space group I222, with unit-cell parameters a = 69.1, b = 72.0, c = 65.4 angstroms. They diffract to 1.35 angstroms resolution using synchrotron radiation. A Matthews coefficient of 2.0 angstroms3 Da(-1) suggests one subunit in the asymmetric unit. Diffraction data for an isomorphous uranium derivative were collected and a difference Patterson map showed strong peaks which allowed determination of the position of the uranium ions.