Rac, a small guanosine triphosphate-binding protein, and p21-activated kinase are activated during platelet spreading on collagen-coated surfaces: roles of integrin alpha(2)beta(1).
Rac, a small guanosine triphosphate-binding protein, and p21-activated kinase are activated during platelet spreading on collagen-coated surfaces: roles of integrin alpha(2)beta(1).
复制标题
Rac（一种小型鸟苷三磷酸结合蛋白）和 p21 激活激酶在血小板在胶原蛋白包被表面上铺展期间被激活：整合素 α(2)β(1) 的作用。
作者：
K. Suzuki;Y. Yatomi;N. Asazuma;M. Kainoh;T. Tanaka;K. Satoh;Y. Ozaki
In this study, the receptors and signals involved in collagen-induced platelet spreading were examined. It was found that platelet spreading on collagen (presenting a polygon shape with a number of filopodialike projections) was inhibited by the anti-integrin alpha(2) antibody, suggesting the involvement of integrin alpha(2)beta(1) in this process. Studies with a glutathione-S-transferase fusion protein that binds specifically to activated Rac and in vitro p21-activated kinase (PAK) kinase assays revealed that Rac and PAK were activated during this collagen-activated process. Platelet spreading on collagen-coated surfaces was inhibited strongly by PP1 (a Src family kinase inhibitor) or weakly by wortmannin (a phosphatidylinositol 3-kinase [PI3-kinase] inhibitor) but not at all by Y-27632 (a Rho kinase inhibitor). The surfaces coated with anti-integrin alpha(2)beta(1) antibodies also induced platelet spreading (presenting an almost complete round shape) and activation of Rac and PAK, although more slowly than collagen-coated surfaces. The antibody-induced responses were strongly inhibited by PP1 or wortmannin but not by Y-27632. The same concentration of Y-27632 inhibited collagen-induced shape change of platelets in suspension. These findings suggest that Rac and/or PAK activation, but not Rho, may play certain roles in platelet spreading via integrin alpha(2)beta(1) and that Src family kinases and PI3-kinase participate in these processes. Furthermore, the difference between spreading on collagen and the anti-integrin antibody suggests the involvement of other receptor(s) (in addition to the integrin alpha(2)beta(1)) for collagen-induced spreading, the most likely candidate being glycoprotein VI.
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影响因子：
20.3
作者：
Azim, AC;Barkalow, K;Hartwig, JH
通讯作者：
Hartwig, JH
DOI：
10.1016/s0021-9258(17)42774-8
发表时间：
1984-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Daniel;I. Molish;M. Rigmaiden;G. Stewart
通讯作者：
J. Daniel;I. Molish;M. Rigmaiden;G. Stewart
影响因子：
3.3
作者：
Price, LS;Leng, J;Bokoch, GM
通讯作者：
Bokoch, GM
影响因子：
20.3
作者：
Falet, H;Barkalow, KL;Hartwig, JH
通讯作者：
Hartwig, JH
在血细胞比容升高时纠正 Delta 存储池缺陷中的血小板粘附缺陷——二磷酸腺苷的可能作用。
影响因子：
20.3
作者：
Weiss,HJ;Lages,B;Hoffmann,T;Turitto,VT
通讯作者：
Turitto,VT