Generation of virus-resistant potato plants by RNA genome targeting
Generation of virus-resistant potato plants by RNA genome targeting
复制标题
通过RNA基因组靶向培育抗病毒马铃薯植株
DOI：
10.1111/pbi.13102
复制
发表时间：
2019
影响因子：
13.8
通讯作者：
Zhang Jiang
中科院分区：
文献类型：
--
作者：
Zhan Xiaohui;Zhang Fengjuan;Zhong Ziyang;Chen Ruhao;Wang Yong;Chang Ling;Bock Ralph;Nie Bihua;Zhang Jiang
CRISPR/Cas系统为细菌和古细菌提供了抵抗入侵噬菌体和外来质粒的分子免疫。2类VI型CRISPR/Cas效应物Cas13a是一种RNA靶向CRISPR效应物，可提供针对RNA噬菌体的保护。在这里，我们报告了CRISPR/Cas13a的重新用途，以保护马铃薯植物免受真核病毒马铃薯病毒Y（PVY）的侵害。表达Cas13a/sgRNA（小向导RNA）结构的转基因马铃薯株系显示PVY积累和疾病症状受到抑制。病毒抗性水平与植物中Cas13a/sgRNA构建体的表达水平相关。我们的数据进一步表明，适当设计的sgrna可以特异性地干扰多种PVY菌株，而对PVA或马铃薯病毒s等不相关的病毒没有影响。我们的发现为工程作物提供了一种新颖而高效的抗病毒策略。
CRISPR/Cas systems provide bacteria and archaea with molecular immunity against invading phages and foreign plasmids. The class 2 type VI CRISPR/Cas effector Cas13a is an RNA‐targeting CRISPR effector that provides protection against RNA phages. Here we report the repurposing of CRISPR/Cas13a to protect potato plants from a eukaryotic virus,Potato virus Y(PVY). Transgenic potato lines expressing Cas13a/sgRNA (small guide RNA) constructs showed suppressed PVY accumulation and disease symptoms. The levels of viral resistance correlated with the expression levels of the Cas13a/sgRNA construct in the plants. Our data further demonstrate that appropriately designed sgRNAs can specifically interfere with multiple PVY strains, while having no effect on unrelated viruses such as PVA orPotato virus S. Our findings provide a novel and highly efficient strategy for engineering crops with resistances to viral diseases.