Active chromatin domains are defined by acetylation islands revealed by genome-wide mapping
Active chromatin domains are defined by acetylation islands revealed by genome-wide mapping
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DOI：
10.1101/gad.1272505
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发表时间：
2005-03-01
影响因子：
10.5
通讯作者：
Zhao, K
中科院分区：
文献类型：
--
作者：
Roh, TY;Cuddapah, S;Zhao, K
人类细胞的身份和发育潜力由其表观基因组指定，表观基因组在很大程度上由包括组蛋白乙酰化在内的染色质修饰模式定义。在这里，我们报告了高分辨率的全基因组定位技术（GMAT）的组蛋白H3在赖氨酸9和赖氨酸14在静息和激活的人类T细胞的双乙酰化。我们的数据表明，高水平的H3乙酰化检测基因丰富的地区。遗传结构域的染色质可及性和基因表达与启动子和其他调控元件的超乙酰化相关，但与整个结构域的乙酰化水平普遍升高无关。在基因间区和转录区发现了乙酰化岛。在这项研究中确定的46，813个乙酰化岛的位置与保守的非编码序列（CNSs）显着相关，其中许多与T细胞中的已知调控元件共定位。TCR信号转导诱导了4045个新的乙酰化位点，这些位点可能介导了整体染色质重塑和基因激活。我们建议，乙酰化岛是表观遗传标记，允许预测的功能调控元件。
The identity and developmental potential of a human cell is specified by its epigenome that is largely defined by patterns of chromatin modifications including histone acetylation. Here we report high-resolution genome-wide mapping of diacetylation of histone H3 at Lys 9 and Lys 14 in resting and activated human T cells by genome-wide mapping technique (GMAT). Our data show that high levels of the H3 acetylation are detected in gene-rich regions. The chromatin accessibility and gene expression of a genetic domain is correlated with hyperacetylation of promoters and other regulatory elements but not with generally elevated acetylation of the entire domain. Islands of acetylation are identified in the intergenic and transcribed regions. The locations of the 46,813 acetylation islands identified in this study are significantly correlated with conserved noncoding sequences (CNSs) and many of them are colocalized with known regulatory elements in T cells. TCR signaling induces 4045 new acetylation loci that may mediate the global chromatin remodeling and gene activation. We propose that the acetylation islands are epigenetic marks that allow prediction of functional regulatory elements.