RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction
RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction
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DOI：
10.1093/bioinformatics/btaa635
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发表时间：
2020-10-01
期刊：
影响因子：
5.8
通讯作者：
Zhang, Hao F.
中科院分区：
文献类型：
--
作者：
Davis, Janel L.;Soetikno, Brian;Zhang, Hao F.
摘要：光谱单分子定位显微镜 (sSMLM) 同时捕获随机发射荧光单分子的空间位置和全光谱。它提供了一个光学平台来开发新的多分子和功能成像功能。虽然一些开源软件套件提供荧光分子的亚衍射定位，但用于 sSMLM 数据光谱分析的软件套件仍然不可用。 RainbowSTORM 是一个开源 ImageJ/FIJI 插件，用于 sSMLM 图像的端到端光谱分析和可视化。 RainbowSTORM 允许用户校准、预览和定量分析使用不同报告的 sSMLM 系统设计和荧光标签获得的发射光谱。
A Summary: Spectroscopic single-molecule localization microscopy (sSMLM) simultaneously captures the spatial locations and full spectra of stochastically emitting fluorescent single molecules. It provides an optical platform to develop new multimolecular and functional imaging capabilities. While several open-source software suites provide subdiffraction localization of fluorescent molecules, software suites for spectroscopic analysis of sSMLM data remain unavailable. RainbowSTORM is an open-source ImageJ/FIJI plug-in for end-to-end spectroscopic analysis and visualization for sSMLM images. RainbowSTORM allows users to calibrate, preview and quantitatively analyze emission spectra acquired using different reported sSMLM system designs and fluorescent labels.