TCF7L2 gene expression in human visceral and subcutaneous adipose tissue is differentially regulated but not associated with type 2 diabetes mellitus
TCF7L2 gene expression in human visceral and subcutaneous adipose tissue is differentially regulated but not associated with type 2 diabetes mellitus
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DOI：
10.1016/j.metabol.2008.04.016
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发表时间：
2008-09-01
影响因子：
9.8
通讯作者：
Blueher, Matthias
中科院分区：
文献类型：
--
作者：
Kovacs, Peter;Berndt, Janin;Blueher, Matthias
TCF7L2基因的变异与2型糖尿病（T2DM）相关，但致病变异仍不清楚。我们研究了TCF7L2信使RNA（mRNA）的表达在配对样本的内脏和皮下脂肪组织前49名受试者使用定量实时聚合酶链反应及其与肥胖和2型糖尿病的关系。对所有受试者进行先前描述的TCF7L2糖尿病风险变异的基因分型。与年龄、性别、肥胖和糖尿病状况无关，我们发现皮下脂肪组织中TCF 7L2 mRNA的表达比内脏脂肪组织高出3倍以上。内脏和皮下TCF7L2表达之间没有相关性。在糖尿病和非糖尿病受试者之间，或者在瘦型和肥胖受试者之间，脂肪组织TCF 7L2 mRNA表达水平没有差异（所有Ps > 0.05）。此外，TCF7L2基因变异与mRNA表达之间没有关联。根据我们的数据，TCF7L2 mRNA表达是脂肪储存特异性的，但似乎没有提供解释与T2DM遗传相关性的机制联系。(c)2008年爱思唯尔公司All rights reserved.
Variants in the TCF7L2 gene have been associated with type 2 diabetes mellitus (T2DM), but the causal variant(s) is still unknown. We studied the TCF7L2 messenger RNA (mRNA) expression in paired samples of visceral and subcutaneous adipose tissue front 49 subjects using quantitative real-time polymerase chain reaction and its relation to obesity and T2DM. All subjects were genotyped for the previously described TCF7L2 diabetes risk variants. Independent of age, sex, obesity, and diabetes status, we found >3-fold higher TCF7L2 mRNA expression in subcutaneous compared with visceral adipose tissue. There was no correlation between visceral and subcutaneous TCF7L2 expression. No differences in adipose tissue TCF7L2 mRNA expression levels were found between diabetic and nondiabetic subjects, or between lean and obese subjects (all Ps >.05). In addition, there was no association between TCF7L2 genetic variants and mRNA expression. Based on our data, TCF7L2 mRNA expression is fat-depot specific but does not seem to provide the mechanistic link explaining genetic association with T2DM. (c) 2008 Elsevier Inc. All rights reserved.