Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.
Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.
复制标题
在小鼠中产生表达种系特异性报告基因的新型种系干细胞系。
作者：
Shiura H;Ikeda R;Lee J;Sato T;Ogonuki N;Hirose M;Ogura A;Ogawa T;Abe K.
生殖系干细胞是由出生后的雄性生殖系细胞衍生而来的干细胞系。值得注意的是，GS细胞移植到不育的宿主小鼠睾丸后可以形成有功能的精子，表明GS细胞具有精原干细胞(SSC)活性。由于GS细胞是唯一具有SSC活性的细胞类型，因此最适合于雄性生殖细胞分化的体外研究。然而，根据培养条件的不同，GS细胞可能会偏离生殖细胞状态，成为其他类型的细胞。因此，希望有一种监控系统来确保GS细胞在培养过程中保持在生殖细胞状态。在这里，我们从转基因小鼠的新生睾丸中建立了GS细胞系，该细胞系表达荧光蛋白Venus，其基因表达受Mvh(鼠Vasahomolog)启动子的驱动，Mvh是哺乳动物生殖细胞高度特异的基因。这种新的细胞系具有与现有GS系相同的真正GS细胞特性，包括产生可存活后代的能力。据我们所知，这是第一个表达生殖细胞特异性报告的Mvh-VenusGS细胞系。这一宝贵的资源将为男性生殖细胞分化的研究提供新的机会。创世纪51：498-505。©2013 Wiley期刊，Inc.
Germline stem (GS) cells are stem cell lines derived from postnatal male germline cells. Remarkably, GS cells can form functional spermatozoa when transplanted into infertile host mouse testes, indicating that GS cells have spermatogonial stem cell (SSC) activity. As GS cells are the only type with SSC activity, they are most suitable for in vitro studies on male germ cell differentiation. However, GS cells can deviate from the germ cell state to become other types of cells, depending on culture conditions. Therefore, it is desirable to have a monitor system to ensure that GS cells are kept at the germ cell state in culture. Here, we established GS cell lines from neonatal testes of transgenic mice that express the fluorescent protein, Venus, whose gene expression is driven by the promoter ofMvh(mouseVasahomolog), a gene highly specific to mammalian germ cells. This novel cell line has genuine GS cell properties equivalent to existing GS lines, including the ability to generate viable offspring. ThisMvh–VenusGS cell line, to our knowledge, is the first one expressing a germ cell‐specific reporter. This valuable resource should provide new opportunities for studies on male germ cell differentiation. genesis 51:498–505. © 2013 Wiley Periodicals, Inc.