Fatty acid profiles during gametogenesis in sea urchin (Paracentrotus lividus): Effects of dietary inputs on gonad, egg and embryo profiles
Fatty acid profiles during gametogenesis in sea urchin (Paracentrotus lividus): Effects of dietary inputs on gonad, egg and embryo profiles
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DOI：
10.1016/j.cbpa.2012.11.010
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发表时间：
2013-02-01
影响因子：
2.3
通讯作者：
Migaud, Herve
中科院分区：
文献类型：
--
作者：
Carboni, Stefano;Hughes, Adam D.;Migaud, Herve
研究了膳食脂肪酸对青色副甲螈性腺组成的影响，以确定膳食输入是否影响其在配子发生过程中的相对丰度。将卵子和胚胎 FA 组成与成熟性腺的 FA 组成进行比较，以了解母体 FA 如何转移给后代。海胆喂食实验性颗粒饲料，与棕色海带（指状昆布）进行比较。饮食、性腺、卵子和胚胎的 FA 谱显示，性腺中存在饮食中不存在的 FA 和/或某些长链多不饱和脂肪酸 (LC-PUFA) 含量较高。此外，在性腺、卵和胚胎中发现了一些不寻常的 FA，例如非亚甲基中断 (NMI)，但在饮食中没有发现，这表明青青青虾可能能够合成这种 FA 并将其积累在卵中。首次报道了配子发生过程中性腺 FA 谱的描述，数据表明二十碳五烯酸和二十二碳六烯酸在配子发生过程中积累，而花生四烯酸受到高度调节，并且是唯一与 NMI 一起明确积累到卵中的 LC-PUFA。需要进一步研究以确定母体提供 FA 是否有可能影响海胆产量并提高孵化场的盈利能力。 (C) 2012 Elsevier Inc. 保留所有权利。
The effects of dietary fatty acids on the composition of Paracentrotus lividus gonads were investigated to termine whether dietary inputs affect their relative abundance during gametogenesis. Egg and embryo FA compositions were compared with that of mature gonads to understand how maternal FA is transferred to the offspring. Urchins were fed an experimental Pellet diet in comparison to brown Kelp (Laminaria digitata). FA profiles of diets, gonads, eggs and embryos revealed the presence in gonads of FA that was absent in the diets and/or higher in contents of some long-chain polyunsaturated fatty acid (LC-PUFA). Moreover, some unusual FA, such as non-methylene interrupted (NMI), was found in gonads, eggs and embryos, but not in the diets, suggesting that P. lividus may be capable of synthesizing this FA and accumulating them in the eggs. A description of gonad FA profiles during gametogenesis is reported for the first time and data suggest that eicosapentaenoic and docosahexaenoic acids are accumulated during gametogenesis, while arachidonic acid is highly regulated and is the only LC-PUFA clearly accumulated into the eggs along with NMI. Further studies are required to determine if maternal provisioning of FA has the potential to influence sea urchin production outputs and to increase hatchery profitability. (C) 2012 Elsevier Inc. All rights reserved.