cDNA microarray analysis of autoimmune hepatitis, primary biliary cirrhosis and consecutive disease manifestation
cDNA microarray analysis of autoimmune hepatitis, primary biliary cirrhosis and consecutive disease manifestation
复制标题
DOI：
10.1016/j.jaut.2005.03.009
复制
发表时间：
2005-09-01
影响因子：
12.8
通讯作者：
Kaneko, S
中科院分区：
文献类型：
--
作者：
Honda, M;Kawai, H;Kaneko, S
虽然自身免疫性肝炎（AlH）和原发性胆汁性肝硬化（PBC）通常具有不同的临床表现，但有些患者同时具有两种情况的特征。利用基因芯片技术，我们分析并比较了8例AlH、9例PBC、8例慢性丙型肝炎（CHC）、8例非酒精性脂肪性肝炎（NASH）和9例正常肝脏患者的基因表达谱。随后，我们将该方法应用于一位具有AlH和PBC重叠特征的61岁女性的组织样本。一名61岁妇女因血清碱性磷酸酶升高而入院。肝活检显示胆管细胞周围有单个核细胞积聚，这是慢性非化脓性破坏性胆管炎（CNSDC）的特征。三年后，她的血清丙氨酸转氨酶（ALT）水平升高，肝脏活检显示有严重肝炎的证据。两个活组织检查的cDNA微阵列分析确定了与组织学改变相关的分子事件。该患者的表达谱从最初不同于其他PBC患者，转变为AIH模式。体外结果提示该患者同时具有AIH和PBC的特征。(c) 2005 Elsevier Ltd版权所有。
While autoimmune hepatitis (AlH) and primary biliary cirrhosis (PBC) usually have distinct clinical manifestations, some patients present with features of both conditions. Using cDNA microarrays, we analyzed and compared gene expression profiles in 8 patients with AlH, 9 with PBC, 8 with chronic hepatitis C (CHC), 8 with non-alcoholic steatohepatitis (NASH) and 9 with normal livers. We subsequently applied this method to a tissue sample from a 61-year-old woman with overlapping features of both AlH and PBC. A 61-year-old woman was admitted to our hospital for evaluation of elevated serum alkaline phosphatase. A liver biopsy showed accumulation of mononuclear cells around the bile duct cells, a feature characteristic of chronic non-suppurative destructive cholangitis (CNSDC). Three years later, her serum alanine aminotransferase (ALT) level had increased, and a liver biopsy demonstrated evidence of a severe form of hepatitis. A cDNA microarray analysis of both biopsies identified the molecular events associated with her altered histology. The expression profile of this patient, which was originally different from that of the other PBC patients, changed to an AIH pattern. Out- results suggest that this patient has characteristics of both AIH and PBC. (c) 2005 Elsevier Ltd. All rights reserved.