Developmental validation of the AmpFlSTR® NGM SElect™ PCR Amplification Kit: A next-generation STR multiplex with the SE33 locus
Developmental validation of the AmpFlSTR® NGM SElect™ PCR Amplification Kit: A next-generation STR multiplex with the SE33 locus
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DOI：
10.1016/j.fsigen.2012.05.012
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发表时间：
2013-01-01
影响因子：
3.1
通讯作者：
Mulero, Julio J.
中科院分区：
文献类型：
--
作者：
Green, Robert L.;Lagace, Robert E.;Mulero, Julio J.
AmpFlSTR（R）NGM SElect（TM）PCR扩增试剂盒是一种新的17重STR基因分型试剂盒，主要用于法医案例分析。该试剂盒被设计成AmpFlSTR（R）NGM（TM）试剂盒的对应物，用于希望将SE 33基因座添加到新的STR基因座欧洲标准集的实验室。NGM SElect Kit与NGM Kit共享16个常见基因座的相同引物组（D10S1248、D3S1358、vWA、D16S539、D2S1338、釉原蛋白、D8S1179、D21S11、D18S51、D19S433、TH 01、FGA、D22S1045、D2S441、D1S1656和D12S391），用SE33基因座的额外引物。开发验证研究遵循STR试剂盒生产商的DNA分析方法科学工作组（SWGDAM）指南，并测试了试剂盒性能的几个关键领域，包括灵敏度系列、DNA混合物和抑制样品。研究表明，NGM SElect Kit提供了与NGM Kit等同的总体性能，但由于包含了高度信息化的SE 33基因座，因此具有更大的区分能力。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
The AmpFlSTR (R) NGM SElect (TM) PCR Amplification Kit is a new 17-plex STR genotyping kit designed for use primarily in forensic casework analysis. The kit was designed to be a counterpart to the AmpFlSTR (R) NGM (TM) Kit for laboratories wishing to add the SE33 locus to the new European Standard Set of STR loci. The NGM SElect Kit shares the same primer sets for 16 common loci with the NGM Kit (D10S1248, D3S1358, vWA, D16S539, D2S1338, amelogenin, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D22S1045, D2S441, D1S1656 and D12S391), with additional primers for the SE33 locus. Developmental validation studies followed the Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines for STR kit manufacturers and tested several critical areas of kit performance including a sensitivity series, DNA mixtures and inhibited samples. The studies demonstrated that the NGM SElect Kit provides equivalent overall performance to the NGM Kit, but with even greater discriminatory power due to the inclusion of the highly informative SE33 locus. (C) 2012 Elsevier Ireland Ltd. All rights reserved.