Prior treatment with vitamin K2 significantly improves the efficacy of risedronate
Prior treatment with vitamin K2 significantly improves the efficacy of risedronate
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DOI：
10.1007/s00198-009-0888-z
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发表时间：
2009-11-01
影响因子：
4
通讯作者：
Sakurai, T.
中科院分区：
文献类型：
--
作者：
Matsumoto, Y.;Mikuni-Takagaki, Y.;Sakurai, T.
先前使用甲烯甲萘醌 MK-4 进行 8 周治疗，随后进行 8 周利塞膦酸盐治疗，可防止个别药物的缺点，并与卵巢切除 (OVX) 对照相比，显着增加卵巢切除 ICR 小鼠股骨的强度。利塞膦酸后服用 MK-4 或同时服用 MK-4 均不推荐，因为它们带来的有益效果最小。本研究的目的是确定利塞膦酸 0.25 mg/kg/天 (R) 与维生素 K-2 约 100 μg MK-4/kg/天 (K) 的最佳组合给药，以提高骨质疏松小鼠骨的强度。 13 周大的 ICR 小鼠，在 9 周时切除卵巢，用 R、K 或 R 加 K (R/K) 治疗 8 周，然后停止治疗 (WO)，或者在 R 和 K 的情况下改用 K 或 R。又过 8 周后，处死小鼠，并通过外周定量计算机断层扫描、微焦点 X 射线管计算机断层扫描和共焦激光拉曼显微光谱进行股骨特性的机械测试和分析。K 至 R 股骨在测试参数（例如材料）方面表现出色。特性、骨矿物质密度、BMC、小梁结构和皮质的几何形状。 K 至 R 中的利塞膦酸盐可防止 K 撤除后发生的横截面惯性矩增加。除了 K 至 R 之外，R 至 WO 骨干和 K 至 WO 骨骺的一些特性显着优于 OVX 对照。与 OVX 对照相比，先用 MK-4 治疗，然后用利塞膦酸盐治疗，股骨强度显着增加。
Prior 8-week treatment with menatetrenone, MK-4, followed by 8-week risedronate prevented the shortcomings of individual drugs and significantly increased the strength of ovariectomized ICR mouse femur compared to the ovariectomized (OVX) controls. Neither MK-4 following risedronate nor the concomitant administration may be recommended because they brought the least beneficial effect.The objective of this study was to determine the best combinatory administration of risedronate at 0.25 mg/kg/day (R) with vitamin K-2 at approximately 100 mu g MK-4/kg/day (K) to improve strength of osteoporotic mouse bone.Thirteen-week-old ICR mice, ovariectomized at 9-week, were treated for 8 weeks with R, K, or R plus K (R/K), and then, either the treatment was withdrawn (WO) or switched to K or R in the case of R and K. After another 8 weeks, the mice were killed, and mechanical tests and analyses of femur properties by peripheral quantitative computed tomography, microfocus X-ray tube computed tomography, and confocal laser Raman microspectroscopy were carried out.The K to R femur turned out superior in parameters tested such as material properties, bone mineral density, BMC, trabecular structure, and geometry of the cortex. The increased cross-sectional moment of inertia, which occurred after K withdrawal, was prevented by risedronate in K to R. In addition to K to R, some properties of R to WO diaphysis and K to WO epiphysis were significantly better than OVX controls.Prior treatment with MK-4 followed by risedronate significantly increased femur strength in comparison to the OVX controls.