Aerobic oxidation of hydrogen sulfide by Thiobacillus denitrificans
Aerobic oxidation of hydrogen sulfide by Thiobacillus denitrificans
复制标题
脱氮硫杆菌有氧氧化硫化氢
DOI：
10.1002/bit.260290605
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发表时间：
1987
影响因子：
3.8
通讯作者：
Kerry L. Subletta
中科院分区：
文献类型：
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作者：
Kerry L. Subletta
已经证明，在硫化物限制条件下，在间歇式和连续流反应器中，在H2S（g）上，可以容易地好氧培养嗜硫杆菌。在这些条件下，培养基中的硫化物浓度小于1 μ M，导致反应器出口气体中的H2S浓度非常低。有氧条件下的生物量产量远低于以前报道的厌氧条件下，大概是因为氧抑制生长。而在45 μ M~150 μ M范围内，稳态氧浓度对生物量没有影响。在0.030 - 0.053 h-1的范围内，生物质产量基本上与比生长速率无关。确定了反应堆失常的指标，并证明了从失常状态的恢复。观察到在有氧条件下用于H2S氧化的生物质的最大负载为15.1 - 20.9 mmol/h/g生物质，这比先前报道的有氧条件高得多。还报道了好氧H2S氧化的化学计量的其他方面。
It has been demonstrated that Thiobacillus denitrificans may be readily cultured aerobically in batch and continuous flow reactors on H2S(g) under sulfide limiting conditions. Under these conditions sulfide concentrations in the culture medium were less than 1μM resulting in very low concentrations of H2S in the reactor outlet gas. Biomass yield under aerobic conditions was much lower than previously reported for anaerobic conditions, presumably because of oxygen inhibition of growth. However, biomass yield was not affected by steady state oxygen concentration in the range of 45μM–150μM. Biomass yield was also observed to be essentially independent of specific growth rate in the range of 0.030–0.053 h−1. Indicators of reactor upset were determined and recovery from upset conditions demonstrated. Maximum loading of the biomass for H2S oxidation under aerobic conditions was observed to be 15.1–20.9 mmol/h/g biomass which is much higher than previously reported for aerobic conditions. Other aspects of the stoichiometry of aerobic H2S oxidation are also reported.