Coexistence of Two blaNDM-5 Genes on an IncF Plasmid as Revealed by Nanopore Sequencing
Coexistence of Two blaNDM-5 Genes on an IncF Plasmid as Revealed by Nanopore Sequencing
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DOI：
10.1128/aac.00110-18
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发表时间：
2018-05-01
影响因子：
4.9
通讯作者：
Zong, Zhiyong
中科院分区：
文献类型：
--
作者：
Feng, Yu;Liu, Lu;Zong, Zhiyong
在167型耐碳青霉烯类大肠杆菌临床分离株中，在F36：A4：B型的144,225个碱基的INCF自传递质粒上发现了两个拷贝的blA(NDM-5)。这两个blaNDM-5基因都位于11,065-bp的区域，两侧是两个拷贝的IS26。这两个区域在序列上是相同的，但存在于质粒上的不同位置，表明相同的区域是重复的。这项研究强调了BLA(NDM-5)的复杂遗传背景。
In a carbapenem-resistant Escherichia coli clinical isolate of sequence type 167, two copies of bla(NDM-5) were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B- type. Both blaNDM-5 genes were located in 11,065-bp regions flanked by two copies of IS26. The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts of bla(NDM-5).