Expression and regulatory effects of microRNA-182 in osteosarcoma cells: A pilot study.
Expression and regulatory effects of microRNA-182 in osteosarcoma cells: A pilot study.
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DOI：
10.3892/ol.2016.4375
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发表时间：
2016-05
期刊：
影响因子：
2.9
通讯作者：
Wu CH
中科院分区：
文献类型：
--
作者：
Bian DL;Wang XM;Huang K;Zhai QX;Yu GB;Wu CH
本研究旨在检测microRNA-182（miRNA-182）在人骨肉瘤MG-63细胞和骨肉瘤组织中的表达水平，探讨miRNA-182对肿瘤生物学活性的影响。本研究采用定量聚合酶链反应（PCR）技术检测了人骨肉瘤MG-63细胞、骨肉瘤组织和正常成骨细胞hFOB1.19中miRNA-182的表达。随后，利用miRNA-182模拟物和抑制剂来调节MG-63细胞中该miRNA的表达水平。采用细胞计数试剂盒-8法检测细胞活力和增殖情况，流式细胞仪检测细胞凋亡情况。采用Transwell小室进行细胞侵袭和迁移实验，分析miRNA-182的体外生物学功能。本研究表明，与hFOB1.19细胞株相比，MG-63细胞和OS组织中miRNA-182的表达水平显著升高（P<0.05）。本研究成功地将miRNA-182抑制剂和miRNA-182模拟物转染到MG-63细胞中，并达到了预期的转染效率。本研究证实，miRNA-182的上调促进细胞凋亡并抑制细胞活力、增殖、侵袭和迁移。本研究进一步证实了miRNA-182是OS中的抑癌基因，因此，调控miRNA-182的表达可能影响OS细胞的生物学行为，提示miRNA-182在恶性肿瘤的分子治疗中具有潜在的作用。
The aim of the present study was to evaluate the expression level of microRNA-182 (miRNA-182) in human osteosarcoma (OS) MG-63 cells and OS tissues, and to elucidate the effect of miRNA-182 on the biological activity of tumors. In the present study, the expression of miRNA-182 in human OS MG-63 cells, OS tissues and normal osteoblast hFOB1.19 cells was determined using quantitative polymerase chain reaction. Subsequently, a miRNA-182 mimic and inhibitor were utilized to regulate the expression level of this miRNA in MG-63 cells. Cell viability and proliferation were examined using cell counting kit-8 assays, and cell apoptosis was detected by flow cytometry. Cell invasion and migration assays were performed using Transwell chambers to analyze the biological functions of miRNA-182 in vitro. The present study demonstrated that the expression level of miRNA-182 in MG-63 cells and OS tissues was significantly increased compared with the hFOB1.19 cell line (P<0.05). The present study successfully performed cell transfections of miRNA-182 inhibitor and miRNA-182 mimic into MG-63 cells and achieved the desired transfection efficiency. The present study confirmed that upregulation of miRNA-182 promotes cell apoptosis and inhibits cell viability, proliferation, invasion and migration. The present findings additionally demonstrated that miRNA-182 is a tumor suppressor gene in OS. Therefore, regulating the expression of miRNA-182 may affect the biological behavior of OS cells, which suggests a potential role for miRNA-182 in molecular therapy for malignant tumors.