Exclusive mutation in epidermal growth factor receptor gene, HER-2, and KRAS, and synchronous methylation of nonsmall cell lung cancer
Exclusive mutation in epidermal growth factor receptor gene, HER-2, and KRAS, and synchronous methylation of nonsmall cell lung cancer
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DOI：
10.1002/cncr.21853
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发表时间：
2006-05-15
期刊：
影响因子：
6.2
通讯作者：
Fujisawa, T
中科院分区：
文献类型：
--
作者：
Suzuki, M;Shigematsu, H;Fujisawa, T
背景非小细胞肺癌（NSCLC）的遗传和表观遗传改变是常见的。分析了表皮生长因子受体基因（EGFR）、HER-2和KRAS的突变以及NSCLC 9个基因的甲基化谱，并与临床和组织学数据进行了相关性分析。在150例NSCLC患者中发现39例EGFR、4例HER-2和6例KRAS突变，基因甲基化百分比为13%-54%。大多数突变存在于腺癌中，但从未发现这3个基因的突变存在于个体肿瘤中。所有基因的甲基化频率与甲基化指数相关，甲基化指数反映了整体甲基化模式（所有基因，P
BACKGROUND. Both genetic and epigenetic changes in nonsmall cell lung cancer (NSCLC) are known to be a common event.METHODS. Mutations in the epidermal growth factor receptor gene (EGFR), HER-2, and KRAS and the methylation profile of 9 genes for NSCLC were analyzed and correlated with clinical and histologic data.RESULTS. Thirty-nine EGFR, 4 HER-2, and 6 KRAS mutations were found in 150 NSCLC cases, with the methylation percentages of the genes ranging from 13% to 54%. Most mutations were present in adenocarcinomas, but mutations of the 3 genes were never found to be present in individual tumors. The frequency of methylation for all the genes was correlated with the Methylation Index, a reflection of the overall methylation pattern (all genes, P