Detection of different developmental stages of malaria parasites by non-radioactive DNAin situ hybridization
Detection of different developmental stages of malaria parasites by non-radioactive DNAin situ hybridization
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非放射性DNA原位杂交检测疟原虫不同发育阶段
DOI：
10.1007/bf01047455
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
B. Mons
中科院分区：
文献类型：
--
作者：
F. M. Berg;P. Amstel;C. Janse;J. Meis;B. Mons
本文描述了一种高度敏感的非放射性脱氧核糖核酸（DNAin）原位杂交程序，可以检测和明确识别人类和啮齿动物疟疾寄生虫的各个发育阶段。使用生物素化的物种特异性DNA探针，红细胞寄生虫可以在血液涂片中特异性染色。在细胞培养和石蜡包埋的肝脏切片中也可以看到类似的红细胞外分期。在血液涂片中，杂交程序提供了快速检测（低）寄生虫和物种确定经验丰富的显微镜在100至400倍的放大。此外，该方法甚至可以在之前的吉姆萨染色后应用，从而可以修改常规吉姆萨染色后难以读取的患者涂片。
A highly sensitive non-radioactive DNAin situhybridization procedure is described that enables detection and unequivocal identification of various developmental stages of human and rodent malaria parasites. Using biotinylated species-specific DNA probes, erythrocytic parasites can be specifically stained in blood smears. Similarly exoerythrocytic stages can be visualized in cell culture and in sections of paraffin-embedded liver. In blood smears, the hybridization procedure provides a rapid detection of (low) parasitemia and species-determination for experienced microscopists at 100 to 400x magnification. Moreover, the procedure can be applied even after previous Giemsa staining of the preparation, enabling revision of patient smears which were difficult to read after routine Giemsa staining.