ISCHEMIA-INDUCED INJURY IN GLYCOGEN-DEPLETED SKELETAL-MUSCLE - SELECTIVE VULNERABILITY OF FG-FIBERS
ISCHEMIA-INDUCED INJURY IN GLYCOGEN-DEPLETED SKELETAL-MUSCLE - SELECTIVE VULNERABILITY OF FG-FIBERS
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DOI：
10.1111/j.1748-1716.1985.tb07776.x
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发表时间：
1985-01-01
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影响因子：
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通讯作者：
JENNISCHE, E
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作者：
JENNISCHE, E
杰尼斯1985.缺血诱导的糖原耗尽骨骼肌损伤。FG纤维的选择性脆弱性。Acta Physiol Scand，125，727 -734。1985年4月16日接收，1985年7月20日接受。ISSN 0001-6772。瑞典哥德堡大学组织学系：在止血带后肢缺血大鼠中研究了缺血前糖原耗竭对骨骼肌损伤发展的影响。在缺血条件下，通过直接电刺激趾长伸肌（EDL）进行糖原消耗。在随后的2.5小时的缺血期间的代谢物负荷通过允许或不允许在电刺激终止后的短的再灌注期来修改。再灌注5或15 h后，通过活体染料排除试验和组织化学染色的组合，从形态学上评估损伤程度，证明钙沉淀物茜素红S。在缺血但未出现糖原耗竭的动物中发现了最小的损伤。在缺血前糖原耗竭的两组动物中，均发生了显著的不可逆损伤。在刺激终止后允许洗脱期的动物中，损伤显著较少。在两种实验条件下，快速糖原分解纤维（FG）对缺血性损伤最敏感，而慢速氧化纤维（SO）则幸免。快速氧化糖原分解纤维（FOG）表现出中等反应。损伤似乎是在再灌注5小时后建立的，这表明在本模型中细胞的反应是全有或全无。
JENNISCHE, E. 1985. Ischaemia‐induced injury in glycogen‐depleted skeletal muscle. Selective vulnerability of FG‐fibres.Acta Physiol Scand, 125,727–734. Received 16 April 1985, accepted 20 July 1985. ISSN 0001–6772. Department of Histology, University of Göteborg, Sweden.The effect of preischaemic glycogen‐depletion on the development of skeletal muscle injury was investigated in rats subjected to tourniquet hind‐limb ischaemia. Glycogen depletion was performed by direct electrical stimulation of the extensor digitorum longus (EDL) muscle during ischaemic conditions. The metabolite load during the subsequent 2.5 h of ischaemia was modified by allowing, or not allowing, a short reperfusion period after termination of the electrical stimulation. The extent of injury was evaluated morphologically after 5 or 15 h of reperfusion, by the combination of an intravital dye exclusion test and a histochemical staining, demonstrating calcium‐precipitates‐Alizarin red S. Minimal damage was found in animals subjected to ischaemia without preceding glycogen depletion. In both groups of preischaemically glycogen‐depleted animals, significant irreversible injury occurred. The injury was significantly less in animals in which a wash‐out period was allowed after termination of stimulation. Fast‐glycogenolytic fibres (FG) were most sensitive to the ischaemic insult during both experimental conditions, while slow‐oxidative fibres (SO) were spared. Fast‐oxidative‐glycogenolytic fibres (FOG) showed an intermediate response. The injury seemed to be established after 5 h of reperfusion, indicating that cells react with an all‐or‐nothing response in the present model.