Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions.
Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions.
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DOI：
10.1016/j.xpro.2021.100563
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发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
Engelhardt B
中科院分区：
文献类型：
--
作者：
Nishihara H;Gastfriend BD;Kasap P;Palecek SP;Shusta EV;Engelhardt B
We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived in vitro blood-brain barrier (BBB) model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion. For complete details on the use and execution of this protocol, please refer to. EECM-BMEC-like cells: a human pluripotent stem cell-derived BBB model Detailed protocol to differentiate human EECM-BMEC-like cells In-depth description of quality control assays for EECM-BMEC-like cells We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived in vitro BBB model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion.
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影响因子：
7.3
作者：
Nishihara, Hideaki;Soldati, Sasha;Engelhardt, Britta
通讯作者：
Engelhardt, Britta
影响因子：
3.7
作者：
Cecchelli R;Aday S;Sevin E;Almeida C;Culot M;Dehouck L;Coisne C;Engelhardt B;Dehouck MP;Ferreira L
通讯作者：
Ferreira L
DOI：
10.1096/fj.202001507rr
发表时间：
2020-12
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Nishihara H;Gastfriend BD;Soldati S;Perriot S;Mathias A;Sano Y;Shimizu F;Gosselet F;Kanda T;Palecek SP;Du Pasquier R;Shusta EV;Engelhardt B
通讯作者：
Engelhardt B
影响因子：
5
作者：
Coisne, C;Dehouck, L;Dehouck, B
通讯作者：
Dehouck, B
影响因子：
5.9
作者：
Lian, Xiaojun;Bao, Xiaoping;Al-Ahmad, Abraham;Liu, Jialu;Wu, Yue;Dong, Wentao;Dunn, Kaitlin K.;Shusta, Eric V.;Palecek, Sean P.
通讯作者：
Palecek, Sean P.