Transport via Macropinocytic Vesicles Is Crucial for Productive Infection with Bombyx Mori Nucleopolyhedrovirus
Transport via Macropinocytic Vesicles Is Crucial for Productive Infection with Bombyx Mori Nucleopolyhedrovirus
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通过大胞饮囊泡的运输对于家蚕核多角体病毒的生产性感染至关重要
DOI：
10.3390/v11070668
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发表时间：
2019-07-01
期刊：
影响因子：
4.7
通讯作者：
Hao, Bifang
中科院分区：
文献类型：
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作者：
Huang, Jinshan;Li, Chenya;Hao, Bifang
家蚕核多角体病毒（Bombyx mori nuclear polyhedrovirus, BmNPV）是蚕业中一种严重的病毒病原体，通过巨噬细胞内吞作用进入宿主细胞；然而，目前对BmNPV进入机制的理解仍然有限。为了确认直接膜融合（DMF）是否会导致BmNPV感染，研究了BmNPV感染期间低pH诱导的DMF感染，并使用egfp标记的病毒粒子追踪感染病毒颗粒。我们发现BmNPV感染有效地诱导液体摄取，这使得BmNPV通过巨噬细胞作用绕过细胞膜屏障。然而，低pH诱导的DMF消除了感染。虽然低pH值是触发膜融合的必要条件，但它并不足以产生BmNPV感染，并且DMF导致无法将核衣壳运输到细胞核中。这些结果表明，通过巨噬细胞囊泡的转运促进了BmNPV进入细胞核，有助于我们了解BmNPV进入细胞核的机制。
Bombyx mori nucleopolyhedrovirus (BmNPV) is a serious viral pathogen in the sericulture industry and enters host cells via macropinocytic endocytosis; however, the current understanding of the BmNPV entry mechanism remains limited. To confirm whether direct membrane fusion (DMF) results in productive BmNPV infection, DMF infectivity induced by low pH during BmNPV infection was investigated, and the infectious viral particle was traced using an eGFP-labeled virion. We found that BmNPV infection efficiently induced fluid uptake, which allowed BmNPV to bypass the cell membrane barrier via macropinocytosis. However, DMF induced by a low pH abolished the infection. While low pH is an essential condition for membrane fusion triggering, it is not sufficient for productive BmNPV infection, and DMF results in failure to transport the nucleocapsid into the nucleus. These results indicate that transport via macropinocytic vesicles facilitates BmNPV entry into the nucleus and contribute to our understanding of the BmNPV entry mechanism.