Metagenomic characterization of the virome associated with bovine respiratory disease in feedlot cattle identified novel viruses and suggests an etiologic role for influenza D virus
Metagenomic characterization of the virome associated with bovine respiratory disease in feedlot cattle identified novel viruses and suggests an etiologic role for influenza D virus
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DOI：
10.1099/jgv.0.000492
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发表时间：
2016-08-01
影响因子：
3.8
通讯作者：
Hause, Ben M.
中科院分区：
文献类型：
--
作者：
Mitra, Namita;Cernicchiaro, Natalia;Hause, Ben M.
牛呼吸道疾病(BRD)是影响养牛业的最昂贵的疾病。BRD的发病机制复杂，包括微生物病原体以及宿主、环境和动物管理因素。在这项研究中，我们利用病毒基因组测序技术对2015年4月至10月分别在墨西哥和美国的6个和4个饲养场采集的急性BRD和无症状饲养牛的鼻拭子样本进行了病毒检测。检出21种病毒，其中牛鼻炎A(52.7%)和B(23.7%)病毒，最常见的是牛冠状病毒(24.7%)。新出现的D型流感病毒与疾病有显著的相关性(P=0.134；优势比=2.94)，而与牛病毒性腹泻病毒、牛疱疹病毒1、牛呼吸道合胞病毒和牛副流感3病毒等常见病毒的检出频率较低。用实时荧光定量聚合酶链式反应进一步证实了IDV的检测。症状动物鼻拭子中IDV RNA含量显著高于健康动物(P=0.04)。除已知的病毒外，还鉴定了牛鼻炎B病毒和肠道病毒E的新基因型别，并鉴定了一种新的猪瘟病毒属--钩状博卡病毒6型。据我们所知，在北美也首次发现了有蹄类四微小病毒1。这些结果说明了与BRD相关的病毒体的复杂性，并强调了进一步研究其他病毒在BRD发病机制中的作用的必要性。
Bovine respiratory disease (BRD) is the most costly disease affecting the cattle industry. The pathogenesis of BRD is complex and includes contributions from microbial pathogens as well as host, environmental and animal management factors. In this study, we utilized viral metagenomic sequencing to explore the virome of nasal swab samples obtained from feedlot cattle with acute BRD and asymptomatic pen-mates at six and four feedlots in Mexico and the USA, respectively, in April-October 2015. Twenty-one viruses were detected, with bovine rhinitis A (52.7 %) and B (23.7 %) virus, and bovine coronavirus (24.7 %) being the most commonly identified. The emerging influenza D virus (IDV) tended to be significantly associated (P= 0.134; odds ratio=2.94) with disease, whereas viruses commonly associated with BRD such as bovine viral diarrhea virus, bovine herpesvirus 1, bovine respiratory syncytial virus and bovine parainfluenza 3 virus were detected less frequently. The detection of IDV was further confirmed using a real-time PCR assay. Nasal swabs from symptomatic animals had significantly more IDV RNA than those collected from healthy animals (P= 0.04). In addition to known viruses, new genotypes of bovine rhinitis B virus and enterovirus E were identified and a newly proposed species of bocaparvovirus, Ungulate bocaparvovirus 6, was characterized. Ungulate tetraparvovirus 1 was also detected for the first time in North America to our knowledge. These results illustrate the complexity of the virome associated with BRD and highlight the need for further research into the contribution of other viruses to BRD pathogenesis.