Coating of titanium alloy with soluble laminin-5 promotes cell attachment and hemidesmosome assembly in gingival epithelial cells: potential application to dental implants.
Coating of titanium alloy with soluble laminin-5 promotes cell attachment and hemidesmosome assembly in gingival epithelial cells: potential application to dental implants.
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DOI：
10.1111/j.1600-0765.1997.tb00536.x
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发表时间：
1997-04
影响因子：
3.5
通讯作者：
R. Tamura;D. Oda;V. Quaranta;G. Plopper;R. Lambert;S. Glaser;J. C. Jones
中科院分区：
文献类型：
--
作者：
R. Tamura;D. Oda;V. Quaranta;G. Plopper;R. Lambert;S. Glaser;J. C. Jones
在牙种植体的经粘膜部分周围形成生物密封对于这些治疗的长期成功可能是至关重要的。迄今为止的数据表明，牙龈上皮附着到牙种植体通过形成半桥粒。生化和遗传数据表明，层粘连蛋白亚型，层粘连蛋白-5，基底膜的一个组成部分，在组装和维持半桥粒中起着至关重要的作用。我们报告使用可溶性层粘连蛋白-5作为钛合金的生物涂层，以促进牙龈上皮细胞系IHGK的细胞附着。与层粘连蛋白-5反应的单克隆抗体耗尽了所有细胞附着活性的包被溶液，并阻断了细胞附着到层粘连蛋白-5包被的盘。用纤维连接蛋白抗体进行免疫耗竭没有效果。最后，我们证明了IHGK细胞在附着层粘连蛋白-5涂层的钛合金24小时内组装半桥粒，但不单独附着钛合金。这些结果表明，可溶性层粘连蛋白-5可能具有作为牙种植体涂层的临床应用，以促进生物密封的形成。
The formation of a biological seal around the transmucosal portion of dental implants may be crucial for the long-term success of these therapies. Data to date suggest that the gingival epithelium attaches to dental implants through the formation of hemidesmosomes. Biochemical and genetic data indicate that the laminin isoform, laminin-5, a component of basement membranes, plays a crucial role in the assembly and maintenance of hemidesmosomes. We report the use of soluble laminin-5 as a biological coating of titanium-alloy to promote cell attachment of the gingival epithelial cell line, IHGK. Monoclonal antibodies reactive with laminin-5 depleted the coating solution of all cell attachment activity and blocked cell attachment to laminin-5-coated disks. Immunodepletion with antibodies to fibronectin had no effect. Finally, we demonstrate that IHGK cells assembled hemidesmosomes within 24 h of attachment to laminin-5-coated titanium alloy but not to the titanium alloy alone. These results suggest that soluble laminin-5 may have clinical applications as a dental implant coating to promote the formation of a biological seal.