Negative effects of a low-quality protein diet on wound healing via modulation of the MMP2 activity in rats
Negative effects of a low-quality protein diet on wound healing via modulation of the MMP2 activity in rats
复制标题
低质量蛋白质饮食通过调节 MMP2 活性对大鼠伤口愈合产生负面影响
DOI：
10.1007/s00726-020-02831-6
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发表时间：
2020
期刊：
影响因子：
3.5
通讯作者：
Oishi Yuichi
中科院分区：
文献类型：
--
作者：
Yamane Takumi;Konno Ryosuke;Iwatsuki Ken;Oishi Yuichi
蛋白质营养不良在很大程度上与愈合过程的延迟或失败有关。然而，饮食蛋白质质量对伤口愈合的影响在很大程度上是未知的。本研究旨在揭示膳食蛋白质质量对大鼠全层皮肤伤口愈合的影响，并阐明其调控机制。正常饲料喂养大鼠一周后，将大鼠分为3组，分别饲喂酪蛋白饲料、谷蛋白饲料和谷蛋白+赖氨酸饲料。与酪蛋白饮食相比，麸质饮食显著降低体重和伤口愈合，但补充赖氨酸可逆转这种效果。谷朊粉组皮肤白细胞数显著高于酪蛋白组。与酪蛋白组相比，谷蛋白组的受伤皮肤组织显示出较低量的胶原蛋白沉积。与酪蛋白组相比，谷朊粉组皮肤基质金属蛋白酶（MMP）2活性和MMP 14 mRNA水平均显著升高。总之，这项研究表明，低质量的蛋白质饮食通过调节大鼠的MMP 2活性对伤口愈合产生负面影响。
Protein malnutrition is largely associated with a delay or failure of the healing process. However, the effect of dietary protein quality on wound healing is largely unknown. This study aimed to reveal the effect of dietary protein quality on wound healing and elucidate the regulatory mechanisms in a rat model of full-thickness cutaneous wounds. Rats were fed a normal diet for a week, and then they were divided into three groups that were fed the following diet for the experimental period: casein diet, gluten diet and gluten + lysine diet. The gluten diet significantly decreased body weight and wound healing compared with the casein diet, but this effect was reversed by supplementation with lysine. The numbers of leukocytes were significantly higher in the skin of the gluten group than those in the casein group. The wounded skin tissues of the gluten group showed lower amounts of collagen deposition compared with that in the casein group. Our results also showed that both matrix metalloproteinase (MMP) 2 activity andMMP14mRNA levels were significantly increased in the skin of the gluten group, compared with the casein group. In summary, this study suggests low-quality protein diets have negative effects on wound healing via modulation of MMP2 activity in rats.