The dynamics of reconstitution of microtubules around the cell center after cooling.
The dynamics of reconstitution of microtubules around the cell center after cooling.
复制标题
冷却后细胞中心周围微管重建的动态。
DOI：
10.1007/bf01175574
复制
发表时间：
1983
影响因子：
6.6
通讯作者：
Chen Ys
中科院分区：
文献类型：
--
作者：
I. Vorobjev;Chen Ys
在间期PE细胞中，冷却（0 ℃下2 h）后，一些微管保留在细胞质中。将细胞转移到恒温器（37摄氏度）后，微管网络的重建开始于细胞中心附近。此时，在中心周围的大多数细胞中，可以看到电子致密的微管会聚灶，然后消失。在37 ℃下培养15 ~ 16 min后，从母中心粒径向发散的微管数量达到最大值，从子中心粒径向发散的微管数量在培养12 min后达到最大值。在加热开始后45分钟，径向发散的微管的数量稍微超过对照水平。这些数据表明，微管仅在其生长期间与中心相关联。成熟的微管与中心分离，并可能被新的微管取代。因此，大多数（不是全部）微管起源于细胞中心，但在任何时候，只有一些微管与它相关联。
In interphase PE cells, after cooling (2 h at 0 degree C), some microtubules are retained in the cytoplasm. After the transfer of the cells to a thermostat (37 degrees C), the reconstitution of the microtubule network begins near the cell center. At this time in most of the cells around the center one can see the electron-dense foci of convergence of microtubules which then disappear. The number of microtubules diverging radially from the mother centriole reaches a maximum after 15 to 16 min, that of microtubules growing from the daughter centriole 12 min after the cells are placed at 37 degrees C. 45 min after the heating started the number of radially diverging microtubules somewhat exceeds the control level. These data show that microtubules are associated with the centers only during their growth. The mature microtubule is separated from the center and may be replaced by a new one. Thus, most, of not all, microtubules originate from the cell center, but at any moment only some of the microtubules are associated with it.