Adriamycin stimulates only the iron ion-induced, NADPH-dependent microsomal alkane formation.
Adriamycin stimulates only the iron ion-induced, NADPH-dependent microsomal alkane formation.
复制标题
阿霉素仅刺激铁离子诱导的 NADPH 依赖性微粒体烷烃的形成。
DOI：
10.1016/0006-2952(82)90101-0
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发表时间：
1982
影响因子：
5.8
通讯作者：
H. Kappus
中科院分区：
文献类型：
--
作者：
H. Muliawan;M. Scheulen;H. Kappus
方法从体重180 ~ 200 g的雄性Wistar大鼠肝脏按标准程序制备微粒体（11）。这些动物免费得到食物（AltrominR）和自来水。为了完全去除血液污染物，匀浆前肝脏灌注0.9% NaCl in H， O。在最近描述的特殊气密烧瓶中进行孵育(8)。tris - kcl缓冲液（11）中5 ml培养液含有1 mg微粒体蛋白/ml、8 mM na -异柠檬酸盐、1 mM NADP和0.05 mg/ml异柠檬酸脱氢酶（12）。在37℃的预孵育期（3min）之前加入阿霉素（阿霉素*盐酸），该药物来自Farmitalia(3)。然后由NADP引发微粒体酶反应。FeC1,* 4H, O （2.4 × 10D5 M）与NADP同时加入（12）。37℃下振荡的孵育混合物上方的气相为不含烷烃的合成空气（80% N,/20 $0,）
Methods Microsomes were prepared from the livers of male Wistar rats of 180-200 g body weight according to standard procedures (11). The animals received food (AltrominR) and tap water ad libitum. TO remove completely blood contaminants, the livers were perfused with 0.9% NaCl in H, O before homogenization. Incubations were carried out in special gas-tight flasks which have recently been described (8). The 5 ml incubation mixture in Tris-KCl-buffer (11) contained 1 mg microsomal protein/ml, 8 mM Na-isocitrate, 1 mM NADP and 0.05 mg/ml isocitrate dehydrogenase (12). Adriamycin (doxorubicin* HCl) which was from Farmitalia (3) was added before the pre-incubation period (3 min) at 37'C. Afterwards the microsomal enzyme reactions were initiated by NADP. FeC1,* 4H, O (2.4 x 10D5 M) was added simultaneously with NADP (12). The gaseous phase above the incubation mixture which was shaken at 37'C was alkane-free synthetic air (80% N,/20 $0,)