Self-correction in tripronucleated human embryos
Self-correction in tripronucleated human embryos
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DOI：
10.1016/j.fertnstert.2011.07.1087
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发表时间：
2011-10-01
影响因子：
6.7
通讯作者：
Escriba, Maria-Jose
中科院分区：
文献类型：
--
作者：
Grau, Noelia;Escrich, Laura;Escriba, Maria-Jose
目的：探讨三原核（TPN）人类胚胎倍性和父母自我校正的发生。设计：实验。设置：研究机构。患者：32个卵胞浆内单精子注射（ICSI-TPN）产生的TPN胚胎和18个常规IVF（IVF-TPN）产生的TPN胚胎。干预：三原核胚胎体外培养6天。对具有>= 6个细胞的那些进行活组织检查以进行倍性分析。研究囊胚的倍性或亲本遗传。在比较胚泡基因组DNA和父母DNA中的多态性位点后，确定异源遗传。三原核起源，活检时的细胞数量，使用荧光原位杂交进行染色体分析，使用聚合酶链反应扩增和测序进行亲本遗传分析，体外发育至囊胚期，结果：ICSI-TPN和IVF-TPN胚胎中有一半胚胎发育为自校正囊胚，而IVF-TPN胚胎中只有1个发育为自校正囊胚。亲代遗传和倍性都不能决定TPN胚胎发育到囊胚期的能力。然而，TPN胚胎自我校正的能力取决于额外原核的亲本来源。（Fertil Steril（R）2011; 96：951-6.（C）2011年由美国生殖医学协会。
Objective: To explore the occurrence of ploidy and parental self-correction in tripronuclear (TPN) human embryos.Design: Experimental.Setting: Research facility.Patient(s): Thirty-two TPN embryos resulting from intracytoplasmic sperm injection (ICSI-TPN) and 18 TPN embryos resulting from conventional IVF (IVF-TPN).Intervention(s): Tripronuclear embryos were cultured in vitro for 6 days. Those with >= 6 cells were biopsied for ploidy analysis. Blastocysts were studied for ploidy or parental inheritance. Heteroparental inheritance was determined after comparing polymorphic loci in the genomic DNA of a blastocyst and in the parents' DNA.Main Outcome Measure(s): Tripronuclear origin, cell number at biopsy, chromosome analysis using fluorescent in situ hybridization, parental inheritance analysis using polymerase chain reaction amplification and sequencing, in vitro development to the blastocyst stage, and percentage of diploid and triploid cleavage embryos and blastocysts.Result(s): Half of ICSI-TPN embryos became self-corrected blastocysts whereas only one IVF-TPN embryo did.Conclusion(s): Both ICSI-TPN and IVF-TPN embryos are capable of self-correction, but the latter to a lesser extent. Neither parental inheritance nor ploidy determines the ability of a TPN embryo to progress to the blastocyst stage. However, the ability of a TPN embryo to become self-corrected is determined by the parental origin of the extra pronucleus. (Fertil Steril (R) 2011; 96: 951-6. (C)2011 by American Society for Reproductive Medicine.)