Cell loss by apoptosis is involved in the intestinal degeneration that occurs during aestivation in the sea cucumber Apostichopus japonicus
Cell loss by apoptosis is involved in the intestinal degeneration that occurs during aestivation in the sea cucumber Apostichopus japonicus
复制标题
细胞凋亡导致的细胞损失与刺参夏眠期间发生的肠道变性有关
DOI：
10.1016/j.cbpb.2017.11.004
复制
发表时间：
--
期刊：
影响因子：
--
通讯作者：
Tianming Wang
中科院分区：
文献类型：
--
作者：
Ke Xu;Qiuhan Yu;Jianshe Zhang;Zhenming Lv;W;ong Fu;Tianming Wang
仿刺参Apostichopus sp.（Selenka）通常会在高温下经历夏眠。这个过程伴随着组织退化和体重减轻。以前的研究表明，细胞凋亡可能在夏眠期间发生的组织重塑中发挥作用，尽管这还没有得到明确的证实。为了验证这一假设，本研究使用了A.作为一种模式生物，以检查细胞损失通过凋亡在肠道退化在夏眠。2016年4月采集自中国黄海（N 36 ° 05 ′ 44.87 ″，E 120 ° 31 ′ 58.51 ″）的仿刺参（Apostichopus）个体，分为两组。实验组在25 ° C孵育诱导夏眠。这导致体重显著降低，苏木精和伊红、Hoechst 33342和组织切片原位TUNEL分析中肠变性证据增加。沿着进一步的Hoechst 33342分析，使用肠细胞涂片，这些结果表明，A.小肠细胞凋亡发生后不久，最初的温度升高，大多数凋亡事件在20天内完成。Ajcaspase-8（CASP8）和Ajcaspase-3（CASP3）凋亡基因的转录定量表明，它们的表达在实验开始时显著升高，但在夏眠后期降低。本研究的结果强烈提示，细胞凋亡参与了A.在理解环境胁迫下组织退化的基本细胞事件中发挥重要作用。
The sea cucumber Apostichopus japonicus (Selenka) commonly undergoes aestivation in response to high water temperatures. This process is accompanied by tissue regression and body mass reduction. Previous studies have suggested that apoptosis may play a role in the tissue remodeling that occurs during aestivation, although this has not definitively been shown. To investigate this hypothesis, the present study used A. japonicus as a model organism to examine cell loss through apoptosis in intestinal degeneration during aestivation. Apostichopus japonicus individuals were collected from Yellow Sea (N 36° 05′ 44.87″, E 120° 31′ 58.51″), China in April 2016 and split into two groups. Aestivation was induced in the experimental group by incubation at 25 °C. This resulted in a significant decrease in body mass and increased evidence of intestinal degeneration in hematoxylin and eosin, Hoechst 33342, and in situ TUNEL analyses of tissue sections. Along with further Hoechst 33342 analysis using intestinal cell smears, these results showed that A. japonicus intestinal cell apoptosis occurred soon after the initial temperature increase, with most apoptotic events completing within 20 days. Transcriptional quantification of the Ajcaspase-8 (CASP8) and Ajcaspase-3 (CASP3) apoptotic genes demonstrated that their expression was significantly elevated at the beginning of the experiment but was decreased at later stages of aestivation. The results of this study strongly suggest that apoptosis is involved in the intestinal regression of A. japonicus during aestivation, and play important role in understanding fundamental cellular events in tissue regression under environmental stress.