Peptide B induction of bag-cell activity in Aplysia: localization of sites of action to the cerebral and pleural ganglia.
Peptide B induction of bag-cell activity in Aplysia: localization of sites of action to the cerebral and pleural ganglia.
复制标题
B 肽对海兔袋细胞活性的诱导：作用部位定位于大脑和胸膜神经节。
DOI：
10.1002/neu.480190804
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Blankenship,JE
中科院分区：
文献类型：
--
作者：
Painter,SD;Rock,MK;Nagle,GT;Blankenship,JE
海洋软体动物的囊细胞是参与产卵起始及其相关行为的模型神经内分泌细胞，但触发囊细胞活动的自然刺激尚不清楚。A.加州是生殖道的外分泌器官，含有两种结构相关的肽（A和B），它们可以在体外诱导后放电，这些肽可以用于探测中枢神经系统，以寻找可能发生外源性兴奋性输入到袋细胞系统的部位。在一系列损伤和消融实验中确定了这些部位。从动物体内取出整个中枢神经系统，并置于具有两个隔室的腔室中，两个隔室可以独立灌注，允许肽（心房腺提取物或纯化肽B）选择性地应用于神经系统的特定区域，同时使用细胞外抽吸电极监测袋细胞活性。当将肽应用于脑神经节、胸膜神经节、胸膜侧结缔组织或10-15%的胸膜内脏结缔组织的吻侧时，只要完整的神经元通路将肽应用部位与袋细胞连接，则持续且可靠地诱导后放电。与此相反，后放电从未观察到的肽时，选择性地施加到颊或足神经节，只有很少观察到的腹神经节和尾侧胸膜内脏连接。这些结果支持以下假设：囊细胞突起和/或对囊细胞突触前兴奋的神经元位于胸膜和大脑神经节中，并缩小了中枢神经系统中可以识别关键启动元件的区域。
The bag cells of the marine molluscAplysiaare model neuroendocrine cells involved in the initiation of egg laying and its associated behaviors, but the natural stimulus triggering bag‐cell activity is not known. The atrial gland ofA. california, an exocrine organ in the reproductive tract, contains two structurally related peptides (A and B) which can induce an afterdischargein vitro, and these peptides can be used to probe the central nervous system for sites where extrinsic excitatory input onto the bag‐cell system might occur. These sites were identified in a series of lesion and ablation experiments. The entire central nervous system was removed from an animal and placed in a chamber with two compartments which could be independently perfused, allowing peptides (atrial gland extract or purified peptide B) to be selectively applied to specific regions of the nervous system while bag‐cell activity was monitored using extracellular suction electrodes. Afterdischarges were consistently and reliably induced when peptides were applied to the cerebral ganglion, the pleural ganglia, the cerebropleural connectives, or the rostral 10–15% of the pleurovisceral connectives, provided that an intact neuronal pathway connected the site of peptide application with the bag cells. In contrast, afterdischarges were never observed when peptides were selectively applied to the buccal or pedal ganglia and only rarely observed when applied to the abdominal ganglion and caudal pleurovisceral connectives. These results support the hypothesis that bag‐cell processes and/or neuron(s) presynaptically excitatory to the bag cells are located in the pleural and cerebral ganglia and narrow the region of the central nervous system where the critical initiator element(s) can be identified.