Regulation of resistance and susceptibility in wheat-powdery mildew pathosystem with exogenous cytokinins
Regulation of resistance and susceptibility in wheat-powdery mildew pathosystem with exogenous cytokinins
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DOI：
10.1016/j.jplph.2009.05.014
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Babosha, Alexander V.
中科院分区：
文献类型：
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作者：
Babosha, Alexander V.
小麦幼苗（Triticum aestivum，品种 Zarya）对白粉病（Erysiphe graminis f）抗性的剂量-反应关系。 sp。小麦马哈尔。 （同义词 Blumeria graminis）是小麦白粉病和外源玉米素的致病生物体，已被研究。两周大的幼苗接种了病原体。接种后立即将玉米素或玉米素核苷添加至营养液中。在大多数情况下，细胞分裂素的剂量反应曲线是多相的，敏感性增加的峰值出现在0.25-1.5和1.5-9μM细胞分裂素处，被0.5-3μM细胞分裂素处的抗性增加的区域分开。矿物营养的变化或同时用噻苯隆治疗揭示了剂量反应曲线的变化，范围从具有最大抵抗力的曲线到具有最大敏感性的曲线。剂量反应的多相性质及其变异性被提出作为早期观察到的细胞分裂素改变抗病性的实验数据差异的可能解释。建议使用串联底物抑制的两个代谢过程的数学模型来解释多相剂量反应。在此模型中，第一个反应的产物用作第二个反应的底物。数值实验显示剂量反应曲线形状的变化以及依赖于细胞分裂素代谢的参数变化。 (C) 2009 爱思唯尔有限公司。版权所有。
Dose-response relationship between resistance of wheat seedlings (Triticum aestivum, cultivar Zarya) to Erysiphe graminis f. sp. tritici Marchal. (Syn. Blumeria graminis), a causal organism of wheat powdery mildew and exogenous zeatin has been investigated. Two-week-old seedlings were inoculated with the pathogen. Zeatin or zeatinriboside were added to the nutrient solution immediately after inoculation. The dose-response curve of cytokinin in the most cases was multiphasic, with peaks of increased susceptibility occurring at 0.25-1.5 and 1.5-9 mu M cytokinin, separated by a region of increased resistance at 0.5-3 mu M cytokinin. The change in mineral nutrition or simultaneous treatment with thidiazuron revealed alterations of the dose-response curve ranging from a curve with maximum of resistance to a curve with maximum of susceptibility. Both multiphase nature of dose-response and its variability were proposed as possible explanations for earlier observed discrepancies in experimental data on modification of disease resistance by cytokinins. A mathematical model for two metabolic processes with substrate inhibition connected in-series was suggested to explain the multiphase dose-response. In this model., the product of the first reaction was used as substrate for the second reaction. Numerical experiments showed the changes in the shape of dose-response curve with changes in parameters dependent of cytokinin metabolism. (C) 2009 Elsevier GmbH. All rights reserved.