Resorbability of bone substitute biomaterials by human osteoclasts
Resorbability of bone substitute biomaterials by human osteoclasts
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DOI：
10.1016/j.biomaterials.2003.10.079
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Amling, M
中科院分区：
文献类型：
--
作者：
Schilling, AF;Linhart, W;Amling, M
第三代生物材料正在设计中，目标是一旦植入，它们将帮助身体自我愈合。这些材料在骨中的一个令人满意的特征是它们具有重塑的能力，即破骨细胞吸收材料，然后通过成骨活动被新形成的骨取代。到目前为止，测试骨替代品这种生物学特性的唯一方法是动物实验，这些实验存在伦理、成本和有限的人体可转移性等所有限制。本研究旨在开发一种人体体外试验，允许在生物材料上直接生成人破骨细胞。以磷酸钙骨水泥和聚甲基丙烯酸甲酯为生物材料进行了验证。用光栅式电子显微镜和计算机辅助图像分析进行定量。以牙本质为内标。我们的测试显示，与不可吸收的PMMA水泥相比，磷酸钙骨水泥具有等量的骨吸收能力。目前的临床矫形实践和未来的骨骼工程都可能受益于评估吸收质量的测试系统的可用性和使用。本文介绍的分析方法可以解决吸收能力的问题，并选择最好的材料作为特定患者的骨替代材料。(C)2003爱思唯尔有限公司。保留所有权利。
Third generation biomaterials are being designed with the aim that once implanted they will help the body to heal itself. One desirable characteristic of these materials in bone is their ability to be remodeled, i.e. that osteoclasts resorb the material and it is subsequently replaced by newly formed bone through osteoblastic activity. So far the only way to test this biological property of bone substitutes are animal experiments with all their limitations like ethics, costs and limited transferability to man. The present study was designed, to develop a human in vitro assay, allowing to generate human osteoclasts directly on the biomaterial. The assay was validated using calcium phosphate cement and PMMA as biomaterials. Quantification was performed by raster electron microscopy and computer assisted image analysis. Dentin was used as internal standard. Our assay shows iso-bone resorbability of calcium phosphate cement in comparison to unresorbable PMMA cement. Both current clinical orthopedic practice and future skeletal engineering may profit from the availability and use of a test system for the assessment of resorption quality. The assay presented here allows to address this question of resorbability and to select the best materials for the use as bone substitutes in specific patients. (C) 2003 Elsevier Ltd. All rights reserved.