Effect of rendering procedures on the scrapie agent
Effect of rendering procedures on the scrapie agent
复制标题
渲染程序对痒病剂的影响
DOI：
10.1136/vr.141.25.643
复制
发表时间：
1997
影响因子：
2.2
通讯作者：
R. J. Cawthorne
中科院分区：
文献类型：
--
作者：
D. Taylor;S. Woodgate;A. Fleetwood;R. J. Cawthorne
一池感染了痒病的羊脑被用来刺穿猪骨和猪肠的混合物。这些数据是在1991年欧盟内部使用的12种绘制程序和3种未使用的绘制程序的试验性摹本中处理的。肉和骨粉，以及脂肪，都是从这些组织中提炼出来的。所有肉和骨粉样品的悬浮液以及两种牛脂样品在小鼠身上进行了痒病传染性检测。这两种牛脂样品都没有可检测到的传染性，但肉和骨粉样品呈阳性，除了那些接触高压蒸汽的过程中产生的样品。此外，采用非典型低温工艺，用热庚烷进行溶剂萃取，制备了痒病原料的颗粒。然后将处理过的木片暴露在蒸汽中以除去残留的溶剂。虽然这些颗粒的初始感染滴度很低，但由于热庚烷和蒸汽处理，似乎没有降低传染性。然而，在非典型低温工艺生产的木屑经高压蒸汽处理后制备的肉骨粉没有检测到传染性。
A pool of scrapie-infected sheep brains was used to spike mixtures of porcine bone and intestine. These were processed in pilot-scale facsimiles of 12 rendering procedures that were in use within the European Union in 1991, and three that were not. Meat and bone meal, and tallow, were produced from the rendered tissues. Suspensions of all the meat and bone meal samples, and two of the tallow samples were assayed in mice for scrapie infectivity. Neither of the tallow samples had any detectable infectivity but the meat and bone meal samples were positive, except for those produced by processes involving exposure to hyperbaric steam. In addition, greaves were produced from the scrapie-spiked raw materials by an atypical low-temperature process and subjected to solvent extraction with hot heptane. The treated greaves were then exposed to steam to drive off residual solvent. Although the starting titre of infectivity in these greaves was low, there appeared to be no reduction in infectivity as a result of the treatments with hot heptane and steam. However, there was no detectable infectivity in the meat and bone meal prepared from the greaves produced by the atypical low-temperature process after it had been exposed to hyperbaric steam.