PARTIAL DESTRUCTION OF BORRELIA-BURGDORFERI WITHIN TICKS THAT ENGORGED ON OSPE-IMMUNIZED OR OSPF-IMMUNIZED MICE
PARTIAL DESTRUCTION OF BORRELIA-BURGDORFERI WITHIN TICKS THAT ENGORGED ON OSPE-IMMUNIZED OR OSPF-IMMUNIZED MICE
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DOI：
10.1128/iai.62.5.2079-2084.1994
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发表时间：
1994-05-01
影响因子：
3.1
通讯作者：
FIKRIG, E
中科院分区：
文献类型：
--
作者：
NGUYEN, TPK;LAM, TT;FIKRIG, E
我们确定伯氏疏螺旋体外表面蛋白（Osps）E和F是否可以引发对莱姆病疫苗有用的免疫应答。B攻毒后30天。在感染后90天获得的小鼠血清中存在针对OspF的抗体。莱姆病患者的血清检查显示，OspF抗体在少数（14%）的早期疾病患者，但在大多数（58%）的晚期疾病患者，而OspE抗体很少检测到的患者。用重组OspE或OspF免疫的小鼠分别产生针对OspE或OspF的高滴度抗体。OspF免疫的小鼠被部分保护免于皮内注射器攻击和蜱介导的B传播。而OspE疫苗接种不赋予免疫力。B。然而，在饱食OspE-（蜱内螺旋体数量与对照相比减少75%）或OspF（蜱内螺旋体数量减少90%）免疫小鼠的蜱内，伯氏菌生物体基本上被破坏。
We determined whether Borrelia burgdorferi outer surface proteins (Osps) E and F could elicit immune responses useful for a Lyme disease vaccine. Thirty days after challenge with B. burgdorferi, mice produced antibodies to OspE but not OspF, whereas antibodies to OspF were present in sera of mice obtained 90 days after infection. Examination of sera from patients with Lyme disease revealed antibodies to OspF in a small number (14%) of early-stage disease patients but in a majority (58%) of patients with late-stage disease, while antibodies to OspE mere rarely detected in patients. Mice immunized with recombinant OspE or OspF produced high titers of antibodies to OspE or OspF, respectively. OspF-immunized mice were partially protected from both intradermal syringe challenge and tick-mediated transmission of B. burgdorferi while vaccination with OspE did not confer immunity. B. burgdorferi organisms were, however, substantially destroyed within ticks that engorged on either OspE- (75% reduction in the number of spirochetes within the ticks, compared with controls) or OspF (90% reduction in the number of spirochetes within the ticks)-immunized mice.