Genistein stimulates hematopoiesis and increases survival in irradiated mice
Genistein stimulates hematopoiesis and increases survival in irradiated mice
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金雀异黄素刺激造血并提高受辐射小鼠的存活率
DOI：
10.1269/jrr.46.425
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发表时间：
2005-12-01
影响因子：
2
通讯作者：
Mi, MT
中科院分区：
文献类型：
--
作者：
Zhou, Y;Mi, MT
成年雄性BALB/c小鼠经口给予金雀异黄素160 mg/kg体重，每日1次，连续7天全身伽玛射线照射，具有辐射防护和促进造血恢复的作用。此外，还比较了染料木素和己烯雌酚的保护作用。通过对照射后小鼠存活率、血象、内源性造血脾集落形成(EndoCFU)和骨髓有核细胞、粒细胞-巨噬细胞集落形成单位(CFU-GM)的研究，证明染料木素具有有效的辐射防护作用。经金雀异黄素保护的受照小鼠的存活率显著增加，且显著高于DES预处理的小鼠。金雀异黄素或DES可刺激小鼠白细胞、红细胞、淋巴细胞和血小板的恢复，但金雀异黄素对骨髓有核细胞、白细胞和淋巴细胞恢复的促进作用明显高于DES。经金雀异黄素和DES处理的小鼠骨髓有核细胞数和CFU-GM也明显增加。同时，金雀异黄素处理组小鼠的EndoCFU数是照射对照组的3.47倍，尽管金雀异黄素组和DES组之间没有显著差异。由此推测，辐射防护作用可能是通过增强造血干细胞的辐射抗性，增加残留的造血细胞数量，促进辐射后的修复或促进造血干细胞的增殖而促进造血干细胞再生的可能过程。金雀异黄素的这些作用可能对放射性损伤有一定的治疗意义。
Radiation protection from death and stimulating hematopoietic recovery by oral administrations of genistein, 160 mg/kg b.w., once daily for seven consecutive days before whole-body gamma-rays irradiation, were confirmed by tests with adult male BALB/c mice. Moreover, the protective action of genistein was compared to that of diethylstilbestrol (DES). Based on the studies of survival, behavior of hematograms, endogenous hematopoietic spleen colony formation (endoCFUs), and numbers of nucleated cell, granulocyte-macrophage colony forming units (CFU-GM) in bone marrow following irradiation, it was demonstrated that genistein was an effective radioprotector. The survival of irradiated mice protected by genistein was significantly increased and statistically higher than that of mice pre-treated with DES. Stimulated recovery of leukocytes, erythrocytes, lymphocytes and thrombocytes were observed in mice pre-treated with genistein or DES, however, the effects of genistein on promoting recovery of bone marrow nucleated cells, leukocytes and lymphocytes were significantly higher than those of DES. Enhanced endoCFUs, numbers of bone marrow nucleated cells and CFU-GM were also found in mice pre-treated with genistein as well as DES. Meanwhile, endoCFU numbers in mice pre-treated with genistein was 3.47-fold higher than that in the irradiated control group, although no significant difference was found between genistein administration and DES administration. It could be deduced that the radioprotective action against death is induced by a possible process of enhanced regeneration of the hematopoietic stem cells due to not only strengthened radioresistance and increased numbers of remained hematopoietic cells, but also enhanced post-irradiation repair or promoted proliferation of the hematopoietic stem cells. These effects of genistein may have some therapeutic implications for radiation-induced injuries.