Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix
Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix
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DOI：
10.1016/j.biomaterials.2018.12.009
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发表时间：
2019-02-01
期刊：
影响因子：
14
通讯作者：
Chen, Shaochen
中科院分区：
文献类型：
--
作者：
Yu, Claire;Ma, Xuanyi;Chen, Shaochen
脱细胞外基质（DEMM）表现出极好的效用，因为在概括复杂的生化微环境中生物镜子，但是，它们用作3D生物印刷的生物互联来产生功能性生物映射组织的使用受到其可调节性和可调节物理性质的限制。在这里，我们描述了一种方法，可以使用基于数字光处理（DLP）的无扫描和连续的3D Bioper来生产具有高度控制复杂微体系结构和机械性能的患者特异性组织，以高度控制患者特异性组织，以产生可光叠链接组织特异性的DECM生物学。我们证明组织匹配的DECM生物互联提供了一个有利的环境，以维持人类诱导的多能干细胞（HIPSC）衍生的心肌细胞和肝细胞的高活性和成熟。微观模式还通过生物物理提示将自发的细胞重组引导为预先设计的条纹心脏和小叶肝结构。我们的方法可以采用基于光的方法，以精确的组织尺度设计快速生物包装DECM生物学，以在生理上具有与生理相关的功能性人体组织来应用生物学，再生医学和诊断。
Decellularized extracellular matrices (dECMs) have demonstrated excellent utility as bioscaffolds in recapitulating the complex biochemical microenvironment, however, their use as bioinks in 3D bioprinting to generate functional biomimetic tissues has been limited by their printability and lack of tunable physical properties. Here, we describe a method to produce photocrosslinkable tissue-specific dECM bioinks for fabricating patient-specific tissues with high control over complex microarchitecture and mechanical properties using a digital light processing (DLP)-based scanningless and continuous 3D bioprinter. We demonstrated that tissue matched dECM bioinks provided a conducive environment for maintaining high viability and maturation of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and hepatocytes. Microscale patterning also guided spontaneous cellular reorganization into predesigned striated heart and lobular liver structures through biophysical cues. Our methodology enables a light -based approach to rapidly bioprint dECM bioinks with accurate tissue-scale design to engineer physiologically-relevant functional human tissues for applications in biology, regenerative medicine, and diagnostics.