Sperm surface fibronectin. Expression following capacitation.
Sperm surface fibronectin. Expression following capacitation.
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DOI：
10.1002/j.1939-4640.1992.tb01623.x
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发表时间：
1992-01
影响因子：
--
通讯作者：
F. Fusi;Richard Bronson
中科院分区：
文献类型：
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作者：
F. Fusi;Richard Bronson
Arg-Gly-Asp（RGD）氨基酸序列在许多细胞-细胞和细胞-基质粘附系统中起作用，作为称为整合素的细胞膜受体的识别序列。整合素VLA亚家族的受体识别纤连蛋白、层粘连蛋白和胶原蛋白。鉴于作者发现纤连蛋白衍生的含RDG的肽在异源（人-仓鼠）和同源（仓鼠-仓鼠）配子相互作用中竞争性抑制精子-卵膜粘附和穿透，研究了新鲜、获能和顶体反应的人精子表面纤连蛋白的表达。大多数新鲜精子在其质膜上不显示纤连蛋白（0 - 16%阳性），这通过缺乏单克隆和多克隆抗纤连蛋白抗体的结合来证明。相比之下，显着更大比例的精子（不同的捐助者之间变化18%至100%）孵育过夜获能条件下与抗纤连蛋白抗体反应。用孕酮诱导顶体反应并不改变精子表面显示纤维连接蛋白的比例或其分布。抗纤连蛋白抗体对人精子的精子膜粘附和仓鼠卵穿透的影响表明纤连蛋白在精子-卵膜相互作用中的生理作用，两者均显著降低（P <0.001）。
The Arg-Gly-Asp (RGD) amino acid sequence plays a role in many cell-to-cell and cell-to-matrix adhesion systems, as a recognition sequence for cell membrane receptors termed integrins. Receptors of the VLA subfamily of integrins recognize fibronectin, laminin, and collagen. Given the authors' findings that fibronectin-derived, RDG-containing peptides competitively inhibit sperm-oolemmal adhesion and penetration in both heterologous (human-hamster) and homologous (hamster-hamster) gamete interactions, the expression of fibronectin on the surface of fresh, capacitated, and acrosome-reacted human spermatozoa was studied. The majority of fresh spermatozoa did not display fibronectin on their plasma membrane (0 to 16% positive), as demonstrated by the lack of binding of both monoclonal and polyclonal anti-fibronectin antibodies. In contrast, a significantly greater proportion of spermatozoa (varying between 18% to 100% for different donors) incubated overnight under capacitating conditions reacted with anti-fibronectin antibodies. The induction of an acrosome reaction with progesterone did not alter the proportion of sperm displaying fibronectin or its distribution on the sperm surface. A physiologic role of fibronectin in sperm-oolemmal interaction was suggested by the effects of anti-fibronectin antibodies on sperm oolemmal adhesion and penetration of hamster eggs by human spermatozoa, which were both significantly reduced (P less than 0.001).