DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis
DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis
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DOI：
10.1093/emboj/cdf657
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发表时间：
2002-12-02
期刊：
影响因子：
11.4
通讯作者：
Fransz, PF
中科院分区：
文献类型：
--
作者：
Soppe, WJJ;Jasencakova, Z;Fransz, PF
我们提出了一个异染色质组装模型，将DNA甲基化与组蛋白甲基化和DNA复制联系起来。以拟南芥低甲基化突变体ddm 1和met 1为材料，研究了DNA甲基化与染色质结构的关系。这两种突变体显示出异染色质的减少，由于分散的pericentromeric低拷贝序列远离异染色质。DDM 1和MET 1通过影响DNA维持（CpG）甲基化和随后的组蛋白H3赖氨酸9甲基化来控制异染色质在染色中心的组装。此外，DDM 1是组蛋白H4赖氨酸16脱乙酰化所必需的。野生型和低甲基化突变体之间的F-1杂交分析表明，DNA甲基化是表观遗传的，代表了维持着丝粒周围异染色质所需的基因组印记。
We propose a model for heterochromatin assembly that links DNA methylation with histone methylation and DNA replication. The hypomethylated Arabidopsis mutants ddm1 and met1 were used to investigate the relationship between DNA methylation and chromatin organization. Both mutants show a reduction of heterochromatin due to dispersion of pericentromeric low-copy sequences away from heterochromatic chromocenters. DDM1 and MET1 control heterochromatin assembly at chromocenters by their influence on DNA maintenance (CpG) methylation and subsequent methylation of histone H3 lysine 9. In addition, DDM1 is required for deacetylation of histone H4 lysine 16. Analysis of F-1 hybrids between wild-type and hypomethylated mutants revealed that DNA methylation is epigenetically inherited and represents the genomic imprint that is required to maintain pericentromeric heterochromatin.