Genome-Wide Analysis of Mitotic Recombination in Budding Yeast.
Genome-Wide Analysis of Mitotic Recombination in Budding Yeast.
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DOI：
10.1007/978-1-0716-0644-5_15
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发表时间：
2021
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DNA断裂损伤对基因组的完整性构成严重威胁。真核细胞可以使用同源重组（HR）途径去除这些病变，该途径通过使用同源DNA模板指导修复反应。芽殖酵母酿酒酵母是一个很好的模型系统，研究这种修复机制和由此产生的基因组变化的模式，从而导致it.In本章中，我们描述了一种方法，利用全基因组测序（WGS）数据来支持的杂合性缺失（洛），可以产生从有丝分裂重组的背景下，整个二倍体酵母基因组的分析道。本章的工作流程和讨论旨在使受过传统训练的分子生物学家和遗传学家在计算方法方面经验有限，能够从概念上理解和执行全基因组洛缺失分析的步骤，并将其应用于自己的特定研究和实验模型。
DNA break lesions pose a serious threat to the integrity of the genome. Eukaryotic cells can remove these lesions using the homologous recombination (HR) pathway that guides the repair reaction by using a homologous DNA template. The budding yeast Saccharomyces cerevisiae is an excellent model system with which to study this repair mechanism and the resulting patterns of genomic change resulting from it. In this chapter, we describe an approach that utilizes whole genome sequencing (WGS) data to support the analysis of tracts of loss-of-heterozygosity (LOH) that can arise from mitotic recombination in the context of the entire diploid yeast genome. The workflow and the discussion in this chapter are intended to enable classically trained molecular biologists and geneticists with limited experience in computational methods to conceptually understand and execute the steps of the genome-wide LOH analysis, as well as to adapt and apply them to their own specific studies and experimental models.