Dysbiosis and compositional alterations with aging in the gut microbiota of patients with heart failure.
Dysbiosis and compositional alterations with aging in the gut microbiota of patients with heart failure.
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DOI：
10.1371/journal.pone.0174099
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Komuro I
中科院分区：
文献类型：
--
作者：
Kamo T;Akazawa H;Suda W;Saga-Kamo A;Shimizu Y;Yagi H;Liu Q;Nomura S;Naito AT;Takeda N;Harada M;Toko H;Kumagai H;Ikeda Y;Takimoto E;Suzuki JI;Honda K;Morita H;Hattori M;Komuro I
新出现的证据表明，肠道微生物群对心力衰竭（HF）的病理生理学具有潜在影响。然而，目前尚不清楚HF是否与肠道微生物群的生态失调有关。我们研究了HF患者肠道微生物群的组成，以阐明肠道微生物生态失调是否与HF相关。我们对12名HF患者和12名年龄匹配的健康对照（HC）受试者的粪便样本进行了16 S核糖体RNA基因测序，并分析了肠道微生物群的差异。我们进一步比较了12名年龄小于60岁的HF患者与10名年龄≥ 60岁的HF患者的肠道微生物群组成。在未加权和加权UniFrac分析中，HF患者的肠道微生物群落组成与HC受试者不同。在HF患者的肠道微生物群中，直肠真杆菌和长链多丽菌的丰度低于HC受试者。与年轻HF患者相比，老年HF患者的拟杆菌门比例降低，变形菌门数量增加。粪杆菌属被耗尽，而乳酸杆菌在老年HF患者的肠道微生物群中富集。这些结果表明，HF患者的肠道微生物群发生了显着变化，并根据年龄进一步变化。心-肠轴的新概念在心力衰竭的诊断和治疗方面有很大的突破潜力。
Emerging evidence has suggested a potential impact of gut microbiota on the pathophysiology of heart failure (HF). However, it is still unknown whether HF is associated with dysbiosis in gut microbiota. We investigated the composition of gut microbiota in patients with HF to elucidate whether gut microbial dysbiosis is associated with HF. We performed 16S ribosomal RNA gene sequencing of fecal samples obtained from 12 HF patients and 12 age-matched healthy control (HC) subjects, and analyzed the differences in gut microbiota. We further compared the composition of gut microbiota of 12 HF patients younger than 60 years of age with that of 10 HF patients 60 years of age or older. The composition of gut microbial communities of HF patients was distinct from that of HC subjects in both unweighted and weighted UniFrac analyses. Eubacterium rectale and Dorea longicatena were less abundant in the gut microbiota of HF patients than in that of HC subjects. Compared to younger HF patients, older HF patients had diminished proportions of Bacteroidetes and larger quantities of Proteobacteria. The genus Faecalibacterium was depleted, while Lactobacillus was enriched in the gut microbiota of older HF patients. These results suggest that patients with HF harbor significantly altered gut microbiota, which varies further according to age. New concept of heart-gut axis has a great potential for breakthroughs in the development of novel diagnostic and therapeutic approach for HF.