Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing
Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing
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DOI：
10.1146/annurev-anchem-061318-115529
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Wang, Tza-Huei
中科院分区：
文献类型：
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作者：
Shin, Dong Jin;Andini, Nadya;Wang, Tza-Huei
面对多重耐药微生物迫在眉睫的威胁，人们越来越需要能够在医疗保健环境中快速识别这些病原体并进行药物敏感性分析的技术。特别是，微流体和核酸扩增方面的最新进展正在突破诊断细菌感染的时间界限。随着分析化学领域技术的多样化和并行发展，需要一个综合的视角来理解这些发展的重要性。本综述探讨了按关键研究领域分组的检测技术新发展的范围。首先，我们研究了细菌感染快速鉴定和药物敏感性测试的核酸扩增测定的最新进展。接下来，我们研究微流体技术的进步，通过集成和规模化促进诊断分析的加速。最后，回顾了生物传感器技术的最新发展。我们以使用新兴概念来开发范式改变分析的观点来结束本次综述。
In the face of looming threats from multi-drug resistant microorganisms, there is a growing need for technologies that will enable rapid identification and drug susceptibility profiling of these pathogens in health care settings. In particular, recent progress in microfluidics and nucleic acid amplification is pushing the boundaries of timescale for diagnosing bacterial infections. With a diverse range of techniques and parallel developments in the field of analytical chemistry, an integrative perspective is needed to understand the significance of these developments. This review examines the scope of new developments in assay technologies grouped by key enabling domains of research. First, we examine recent development in nucleic acid amplification assays for rapid identification and drug susceptibility testing in bacterial infections. Next, we examine advances in microfluidics that facilitate acceleration of diagnostic assays via integration and scale. Lastly, recent developments in biosensor technologies are reviewed. We conclude this review with perspectives on the use of emerging concepts to develop paradigm-changing assays.