Sperm ultrastructure and meiotic segregation in a group of patients with chronic hepatitis B and C
Sperm ultrastructure and meiotic segregation in a group of patients with chronic hepatitis B and C
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DOI：
10.1111/j.1439-0272.2008.00855.x
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发表时间：
2008-10-01
期刊：
影响因子：
2.4
通讯作者：
Collodel, G.
中科院分区：
文献类型：
--
作者：
Moretti, E.;Federico, M. G.;Collodel, G.
关于慢性乙型肝炎B和丙型肝炎对精子质量的影响知之甚少。在这项研究中，我们分析了慢性B型肝炎病毒（HBV）或丙型肝炎病毒（HCV）感染患者的精子质量。精液样本进行了检查，光镜和透射电镜（TEM）。TEM数据用一个数学公式来阐述，该公式能够指示生育指数和三种主要精子病理的存在：细胞凋亡、不成熟和坏死。用18号染色体、X和Y的探针对精子核进行荧光原位杂交，研究减数分裂染色体的分离。尽管精子浓度正常，但我们观察到活力降低。TEM分析强调，35.7%的患者表现出良好的精液质量。然而，与对照组相比，观察到细胞凋亡和坏死的值显著较高，表明精子发生改变。关于减数分裂分离，我们发现二体的发生率与对照组相似，而二倍体导致HCV患者更高，但没有达到统计学意义。总之，研究组的精子质量没有受损;然而，细胞凋亡和坏死超出了正常范围，HCV和HBV感染患者的生育指数显著低于对照组。
Little is known about the effect of chronic hepatitis B and hepatitis C on sperm quality. In this study, we analysed sperm quality from selected patients with chronic hepatitis B virus (HBV) or hepatitis C virus (HCV) infections. Semen samples were examined by light and transmission electron microscopy (TEM). TEM data were elaborated with a mathematical formula able to indicate a fertility index and the presence of the three main sperm pathologies: apoptosis, immaturity and necrosis. Meiotic chromosome segregation was investigated by fluorescence in situ hybridisation carried out on sperm nuclei, using probes for chromosomes 18, X and Y. Despite normal sperm concentration, we observed reduced motility. TEM analysis highlighted that 35.7% of patients showed generally good semen quality. However, significantly higher values of apoptosis and necrosis, compared with controls, were observed, demonstrating spermatogenetic alterations. Regarding meiotic segregation, we found an incidence of disomies similar to that observed in control samples, whereas diploidy resulted higher in HCV patients, without reaching statistical significance. In conclusion, sperm quality in the studied group was not impaired; however, apoptosis and necrosis resulted out of normal range and the fertility index was significantly lower in HCV- and HBV-infected patients versus controls.