Salt taste responses of mouse chorda tympani neurons: Evidence for existence of two different amiloride-sensitive receptor components for NaCl with different temperature dependencies
Salt taste responses of mouse chorda tympani neurons: Evidence for existence of two different amiloride-sensitive receptor components for NaCl with different temperature dependencies
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DOI：
10.1152/jn.1996.76.5.3550
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发表时间：
1996-11-01
影响因子：
2.5
通讯作者：
Ninomiya, Y
中科院分区：
文献类型：
--
作者：
Ninomiya, Y
1.阿米洛利对航行的抑制作用：在两种不同温度（类似于12℃和24℃）下检测C57BL/6品系小鼠鼓索神经单纤维的反应。2．在 36 条对 NaCl 有反应的单纤维中，20 条纤维显示出对阿米洛利舌部治疗引起的 NaCl 反应的强烈抑制（阿米洛利敏感纤维），而其余 16 条纤维则没有表现出这种阿米洛利抑制（阿米洛利不敏感纤维）。 3．二十根阿米洛利敏感纤维根据其 NaCl 响应的温度依赖性进一步分为两个亚组。在 20 根纤维中的 15 根中，24 摄氏度时阿米洛利抑制的 NaCl 反应比 12 摄氏度时更大，而其余 5 根纤维则相反。所有阿米洛利不敏感纤维在 12 摄氏度时对 NaCl 的反应较小。4。这些结果表明，小鼠味觉细胞中存在两种不同的阿米洛利敏感的氯化钠受体成分，具有不同的温度依赖性，一种在较高温度下对氯化钠更敏感，另一种在较低温度下对氯化钠更敏感。
1. Inhibitory effects of amiloride on sail: responses of single fibers of the chorda tympani nerve of the C57BL/6 strain of mice were examined at two different temperatures (similar to 12 and 24 degrees C).2. Of 36 single fibers that responded to NaCl, 20 fibers showed strong suppression of responses to NaCl actuated by lingual treatment with amiloride (amiloride-sensitive fibers), whereas the remaining 16 fibers showed no such amiloride inhibition (amiloride-insensitive fibers).3. Twenty amiloride-sensitive fibers were further classified into two subgroups according to the temperature dependency of their NaCl responses. In 15 of 20 fibers, amiloride-inhibitable NaCl responses were larger at 24 degrees C than at 12 degrees C, whereas the reverse was true for the remaining 5 fibers. All amiloride-insensitive fibers showed smaller responses to NaCl at 12 degrees C.4. These results suggest that there exist, two different amiloride-sensitive receptor components for NaCl with different temperature dependencies in mouse taste cells one is more sensitive to NaCl at the higher temperature, and the other is more sensitive at the lower temperature.